Lazutka J R, Dedonyte V
Department of Botany & Genetics, Vilnius University, Lithuania.
Int J Radiat Biol. 1995 Jun;67(6):671-6. doi: 10.1080/09553009514550791.
Sister chromatid exchanges (SCEs) were analysed in lymphocytes from 12 control persons and 33 Chernobyl clean-up workers. The group of Chernobyl clean-up workers consisted of civilians who were forced to go to Chernobyl to clean up environmental contamination caused by Chernobyl disaster. On average, they received 0.13 (range 0.04-0.249) Gy of external irradiation before returning to home. Cytogenetic analyses were performed 6-8 years after the irradiation. Standard cytogenetic techniques were used. Mean SCE frequency was 7.45 +/- 0.69 SCE/cell in controls and 10.30 +/- 0.31 SCE/cell in clean-up workers (p < 0.05). Analysis of variance showed that exposure to radiation explained 19.6%, occupational exposure to various chemical substances, 11.9%, coffee consumption, 8.3%, smoking, 4.2%, interaction between smoking and coffee consumption, 3.6%, and alcohol abuse, 3.4% of total variation in SCE frequency. Effects of all above confounding factors were significant (p < 0.05). In addition, increased frequencies of chromosome aberrations due to exposure at Chernobyl and alcohol consumption were observed. However, there was no correlation between external dose of irradiation and the frequency of chromosome aberrations. Thus, even 6-8 years after the irradiation, cytogenetic effects in lymphocytes of Chernobyl clean-up workers are still significant.
对12名对照人员和33名切尔诺贝利清理工人的淋巴细胞进行了姐妹染色单体交换（SCE）分析。切尔诺贝利清理工人群体由被迫前往切尔诺贝利清理切尔诺贝利灾难造成的环境污染的平民组成。他们在返回家园之前平均接受了0.13（范围0.04 - 0.249）戈瑞的外照射。在照射后6 - 8年进行了细胞遗传学分析。使用了标准的细胞遗传学技术。对照组的平均SCE频率为7.45±0.69次SCE/细胞，清理工人为10.30±0.31次SCE/细胞（p<0.05）。方差分析表明，辐射暴露解释了SCE频率总变异的19.6%，职业性接触各种化学物质解释了11.9%，咖啡饮用解释了8.3%，吸烟解释了4.2%，吸烟与咖啡饮用之间的相互作用解释了3.6%，酒精滥用解释了3.4%。上述所有混杂因素的影响均具有显著性（p<0.05）。此外，观察到由于在切尔诺贝利的暴露和酒精饮用导致染色体畸变频率增加。然而，外照射剂量与染色体畸变频率之间没有相关性。因此，即使在照射后6 - 8年，切尔诺贝利清理工人淋巴细胞中的细胞遗传学效应仍然显著。
