• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

模拟非洲爪蟾皮肤内蛋白酶识别的RXVRG共有序列的十四肽的结构测定：一种基于模拟退火和一维核磁共振的方法

Structure determination of a tetradecapeptide mimicking the RXVRG consensus sequence recognized by a Xenopus laevis skin endoprotease: an approach based on simulated annealing and 1H NMR.

作者信息

Meddeb S, Chalaoux F R, Ballini J P, Baron D, Vigny P, Demaret J P

机构信息

L.P.C.B., Institut Curie et Université Paris VI, France.

出版信息

J Comput Aided Mol Des. 1995 Apr;9(2):160-70. doi: 10.1007/BF00124406.

DOI:10.1007/BF00124406
PMID:7608747
Abstract

The tetradecapeptide of sequence H-Asp-Val-Asp-Glu-Arg5-Asp-Val-Arg-Gly9-Phe-Ala-Ser-Phe-Leu- NH2 is recognized by a putative maturation endoprotease of the Xenopus laevis skin, which cleaves between Arg8 and Gly9. A conformational search has been performed on this peptide by simulated annealing calculations. Two different models in agreement with the NMR data were found. The conformational difference between the two types of model is located in the consensus sequence, i.e., from Arg5 to Gly9.

摘要

序列为H-Asp-Val-Asp-Glu-Arg5-Asp-Val-Arg-Gly9-Phe-Ala-Ser-Phe-Leu-NH2的十四肽可被非洲爪蟾皮肤中一种假定的成熟内切蛋白酶识别，该酶在Arg8和Gly9之间切割。通过模拟退火计算对该肽进行了构象搜索。发现了两种与核磁共振数据一致的不同模型。两种模型类型之间的构象差异位于共有序列中，即从Arg5到Gly9。

相似文献

1
Structure determination of a tetradecapeptide mimicking the RXVRG consensus sequence recognized by a Xenopus laevis skin endoprotease: an approach based on simulated annealing and 1H NMR.模拟非洲爪蟾皮肤内蛋白酶识别的RXVRG共有序列的十四肽的结构测定：一种基于模拟退火和一维核磁共振的方法
J Comput Aided Mol Des. 1995 Apr;9(2):160-70. doi: 10.1007/BF00124406.
2
Conformational study based on simulated annealing and 1H NMR of peptides comprising the consensus sequence Arg5-Asp-Val-Arg-Gly9: effects of the substitution Ser 12 by Ala 12 or of 3 residues deletion at the N-terminus.基于模拟退火和包含共有序列Arg5-Asp-Val-Arg-Gly9的肽段的1H NMR的构象研究：Ser 12被Ala 12取代或N端缺失3个残基的影响
J Biomol Struct Dyn. 1996 Feb;13(4):661-9. doi: 10.1080/07391102.1996.10508878.
3
Xenopus laevis skin Arg-Xaa-Val-Arg-Gly-endoprotease. A highly specific protease cleaving after a single arginine of a consensus sequence of peptide hormone precursors.
J Biol Chem. 1989 Sep 5;264(25):14609-12.
4
Conformational studies of an undecapeptide reproducing the consensus sequence around the cleavage site of the RXVRG endoprotease from Xenopus laevis skin.对一种十一肽的构象研究，该十一肽再现了非洲爪蟾皮肤中RXVRG内切蛋白酶切割位点周围的共有序列。
Biopolymers. 1994 Oct;34(10):1419-31. doi: 10.1002/bip.360341013.
5
NMR conformational study of a model tetradecapeptide mimicking the RXVRG consensus cleavage site of a Xenopus laevis skin endoprotease.对模拟非洲爪蟾皮肤内蛋白酶RXVRG共有切割位点的十四肽模型进行核磁共振构象研究。
Int J Pept Protein Res. 1995 Jun;45(6):519-28. doi: 10.1111/j.1399-3011.1995.tb01315.x.
6
Two-dimensional 1H NMR study of a tetradecapeptide with the consensus sequence Arg5-Asp-Val-Arg-Gly9: structural effects of the outside substitution Ser12 by Ala12.
J Biomol Struct Dyn. 1995 Apr;12(5):993-1008. doi: 10.1080/07391102.1995.10508792.
7
High-resolution NMR studies of fibrinogen-like peptides in solution: structural basis for the bleeding disorder caused by a single mutation of Gly(12) to Val(12) in the A alpha chain of human fibrinogen Rouen.溶液中纤维蛋白原样肽的高分辨率核磁共振研究：人纤维蛋白原鲁昂Aα链中Gly(12)突变为Val(12)的单一突变导致出血性疾病的结构基础。
Biochemistry. 1989 Apr 4;28(7):3106-19. doi: 10.1021/bi00433a054.
8
Isolation and properties of a multicatalytic proteinase complex from Xenopus laevis skin secretion.非洲爪蟾皮肤分泌物中多催化蛋白酶复合体的分离与特性
Braz J Med Biol Res. 1994 Dec;27(12):2863-7.
9
High-resolution NMR studies of fibrinogen-like peptides in solution: structure of a thrombin-bound peptide corresponding to residues 7-16 of the A alpha chain of human fibrinogen.溶液中纤维蛋白原样肽的高分辨率核磁共振研究：与人纤维蛋白原Aα链7-16位残基对应的凝血酶结合肽的结构
Biochemistry. 1989 Apr 4;28(7):3094-105. doi: 10.1021/bi00433a053.
10
Primary structure of murine major histocompatibility complex alloantigens: amino acid sequence of the amino-terminal one hundred and seventy-three residues of the H-2Kb glycoprotein.小鼠主要组织相容性复合体同种异体抗原的一级结构：H-2Kb糖蛋白氨基末端173个残基的氨基酸序列。
Biochemistry. 1980 Jan 22;19(2):306-15. doi: 10.1021/bi00543a009.

本文引用的文献

1
Optimization by simulated annealing.模拟退火优化。
Science. 1983 May 13;220(4598):671-80. doi: 10.1126/science.220.4598.671.
2
Crystallographic R factor refinement by molecular dynamics.利用分子动力学进行晶体学 R 因子精修。
Science. 1987 Jan 23;235(4787):458-60. doi: 10.1126/science.235.4787.458.
3
Novel peptide fragments originating from PGLa and the caerulein and xenopsin precursors from Xenopus laevis.源自非洲爪蟾的PGLa、蛙皮素和爪蟾素前体的新型肽片段。
J Biol Chem. 1986 Apr 25;261(12):5341-9.
4
Precursors for peptide hormones share common secondary structures forming features at the proteolytic processing sites.肽类激素的前体在蛋白水解加工位点具有共同的二级结构形成特征。
FEBS Lett. 1986 Oct 20;207(1):1-6. doi: 10.1016/0014-5793(86)80002-3.
5
Structural requirement at the cleavage site for efficient processing of the lipoprotein secretory precursor of Escherichia coli.大肠杆菌脂蛋白分泌前体高效加工时切割位点的结构要求。
J Biol Chem. 1986 Aug 25;261(24):10970-5.
6
Signal peptidases recognize a structural feature at the cleavage site of secretory proteins.信号肽酶识别分泌蛋白切割位点处的结构特征。
J Biol Chem. 1988 Jul 25;263(21):10224-8.
7
Determination of three-dimensional structures of proteins by simulated annealing with interproton distance restraints. Application to crambin, potato carboxypeptidase inhibitor and barley serine proteinase inhibitor 2.通过具有质子间距离约束的模拟退火法测定蛋白质的三维结构。应用于胰凝乳蛋白酶原、马铃薯羧肽酶抑制剂和大麦丝氨酸蛋白酶抑制剂2。
Protein Eng. 1988 Apr;2(1):27-38. doi: 10.1093/protein/2.1.27.
8
Xenopus laevis skin Arg-Xaa-Val-Arg-Gly-endoprotease. A highly specific protease cleaving after a single arginine of a consensus sequence of peptide hormone precursors.
J Biol Chem. 1989 Sep 5;264(25):14609-12.
9
Peptides from frog skin.
Annu Rev Biochem. 1990;59:395-414. doi: 10.1146/annurev.bi.59.070190.002143.
10
Endopeptidases and prohormone processing.
Biosci Rep. 1990 Feb;10(1):1-13. doi: 10.1007/BF01116845.