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精子制备方法的比较。洗涤与浓缩、上游法、迁移-重力沉降法、80% Percoll法和精液过滤柱法。

Comparison of sperm preparation methods. Wash and concentration, swim-up, migration-gravity sedimentation, 80% Percoll and semen filtration column.

作者信息

Matsuoka I, Fujino Y, Ito F, Koh B, Kojima T, Ogita S

机构信息

Department of Obstetrics and Gynecology, Osaka City University Medical School, Japan.

出版信息

J Reprod Med. 1995 May;40(5):342-6.

PMID:7608872
Abstract

The removal of all debris and background, as well as antifertility factors present in seminal fluid, may increase the fertilizing ability of spermatozoa. A number of semen manipulative techniques are currently available to remove the undesirable spermatozoa, debris and other factors and to increase sperm quality before performance of the various assisted reproductive technologies. We compared the impact of the wash and concentration method, swim-up method, 80% Percoll method, migration-gravity sedimentation method and SpermPrep method on postmanipulation sperm quality.

摘要

去除所有碎片、背景物质以及精液中存在的抗生育因子,可能会提高精子的受精能力。目前有多种精液处理技术可用于去除不良精子、碎片和其他因素,并在进行各种辅助生殖技术之前提高精子质量。我们比较了洗涤浓缩法、上游法、80% Percoll 法、迁移重力沉降法和 SpermPrep 法对处理后精子质量的影响。

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