Meyer E, Wiegand P, Rand S P, Kuhlmann D, Brack M, Brinkmann B
Institut für Rechtsmedizin, Westfälische Wilhelms-Universität Münster, Germany.
J Mol Evol. 1995 Jul;41(1):10-4. doi: 10.1007/BF00174036.
We amplified, via PCR, DNA segments from intron 1 of the tyrosine hydroxylase gene (TH01) and intron 40 of the von Willebrand factor gene (VWA) in ten nonhuman primate genera. In humans both introns contain polymorphic microsatellites with tetrameric repeats. Compared to the allelic ranges in human populations relatively short repeat arrays could be detected for the nonhuman primates typed, presumably reflecting an ancient precursor state at both microsatellite loci. Furthermore, our results provide evidence for an association of the average number of repeats present in different primate genera and their divergence time from man. DNA sequencing of VWA orthologues revealed a relatively high variability in the arrangement of repeats in the 5'-repeat arrays, the generation of which could probably be explained by polar mutational events.
我们通过聚合酶链反应（PCR）扩增了十种非人类灵长类动物属中酪氨酸羟化酶基因（TH01）内含子1和血管性血友病因子基因（VWA）内含子40的DNA片段。在人类中，这两个内含子都包含具有四聚体重复序列的多态微卫星。与人类群体中的等位基因范围相比，在所检测的非人类灵长类动物中可检测到相对较短的重复阵列，这可能反映了两个微卫星位点的古老前体状态。此外，我们的结果为不同灵长类动物属中存在的重复序列平均数与其与人的分歧时间之间的关联提供了证据。VWA直系同源基因的DNA测序显示，5'-重复阵列中重复序列的排列存在相对较高的变异性，其产生可能可以用极性突变事件来解释。
