Cerezo J R, Jiménez F, Moya F
Departamento de Genética y Microbiología, Universidad de Alicante, San Juan, Spain.
Brain Res Mol Brain Res. 1995 Apr;29(2):245-52. doi: 10.1016/0169-328x(94)00254-c.
A monoclonal antibody, mAb 44D5, has been used to identify and clone Drosophila syntaxin 1 (Dsynt1), an homologue of rat syntaxin 1. The deduced amino acid sequence of the Dsynt1 cDNA cloned is highly homologous to rat syntaxin 1A. Dsynt1 contains 291 amino acid residues and like other members of the syntaxin family is an integral membrane protein, with a transmembrane region at its carboxy-terminus and several regions of the molecule predicted to be in a coiled-coil conformation. The protein is specific to the nervous system and localized in synaptic areas of both central nervous system (CNS) and neuromuscular junction. The same antibody used to clone Dsynt1 cDNA stains synaptic areas in rat cerebellum and a neurospecific antigen in rat and human tissues with identical relative mobility to rat syntaxin 1.
一种单克隆抗体mAb 44D5已被用于鉴定和克隆果蝇 syntaxin 1（Dsynt1），它是大鼠 syntaxin 1的同源物。所克隆的Dsynt1 cDNA推导的氨基酸序列与大鼠 syntaxin 1A高度同源。Dsynt1含有291个氨基酸残基，与 syntaxin家族的其他成员一样，是一种整合膜蛋白，在其羧基末端有一个跨膜区域，并且分子的几个区域预计处于卷曲螺旋构象。该蛋白对神经系统具有特异性，定位于中枢神经系统（CNS）和神经肌肉接头的突触区域。用于克隆Dsynt1 cDNA的同一抗体可对大鼠小脑的突触区域进行染色，并且在大鼠和人类组织中可检测到一种神经特异性抗原，其相对迁移率与大鼠 syntaxin 1相同。
