Fan L, Young P R, Barone F C, Feuerstein G Z, Smith D H, McIntosh T K
Division of Neurosurgery, University of Pennsylvania, Philadelphia 19104, USA.
Brain Res Mol Brain Res. 1995 May;30(1):125-30. doi: 10.1016/0169-328x(94)00287-o.
Cytokines have been shown to be induced following a variety of central nervous system (CNS) insults, and may play a role in the pathophysiological sequelae of CNS injury. In the present study, we characterized the regional expression of interleukin-1 beta (IL-1 beta) mRNA in specific brain regions following experimental lateral fluid-percussion traumatic brain injury (TBI) in rats. Adult Sprague-Dawley rats (n = 42) were anesthetized with sodium pentobarbital (60 mg/kg, i.p.) and subjected to lateral fluid-percussion brain injury of moderate severity (2.4 atm.) centered over the left parietal cortex, or 'sham' treatment (anesthesia and surgery without injury). Animals were sacrificed at 1, 6 and 24 h post injury, brains were removed, and tissue samples of left (injured) parietal cortex (LC), corresponding area in the contralateral right cortex (RC), cortex adjacent to injured parietal cortex (LA), corresponding adjacent area in the right cortex (RA), left hippocampus (LH) and right hippocampus (RH) were prepared. Total RNA was isolated and Northern blot hybridization was performed and the quantity of brain tissue IL-1 beta mRNA is presented as percent relative radioactivity of IL-1 beta positive macrophage RNA which was loaded on same gel.(ABSTRACT TRUNCATED AT 250 WORDS)
细胞因子已被证实在多种中枢神经系统（CNS）损伤后被诱导产生，并且可能在CNS损伤的病理生理后遗症中发挥作用。在本研究中，我们对大鼠实验性侧方流体冲击性脑损伤（TBI）后特定脑区中白细胞介素-1β（IL-1β）mRNA的区域表达进行了表征。成年Sprague-Dawley大鼠（n = 42）用戊巴比妥钠（60 mg/kg，腹腔注射）麻醉，然后接受以左顶叶皮质为中心的中度严重程度（2.4个大气压）的侧方流体冲击性脑损伤，或“假手术”处理（麻醉和手术但无损伤）。在损伤后1、6和24小时处死动物，取出大脑，并制备左（损伤）顶叶皮质（LC）、对侧右皮质相应区域（RC）、损伤顶叶皮质相邻的皮质（LA）、右皮质相应相邻区域（RA）、左海马（LH）和右海马（RH）的组织样本。分离总RNA并进行Northern印迹杂交，脑组织IL-1β mRNA的量以加载在同一凝胶上的IL-1β阳性巨噬细胞RNA的相对放射性百分比表示。（摘要截短于250字）
