Tanaka K, Ohnishi S, Kishimoto N, Kawasaki T, Baba T
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Plant Physiol. 1995 Jun;108(2):677-83. doi: 10.1104/pp.108.2.677.
A rice (Oryza sativa L.) genomic clone encoding the gene for a form of soluble starch synthase (SSS1) and its 5'- and 3'-flanking regions has been isolated and sequenced. The SSS1 gene contained 15 exons interrupted by 14 introns. The exon/intron organization of the SSS1 gene was divergent from that of the rice Waxy gene coding for granule-bound starch synthase, thus suggesting that the SSS1 and granule-bound starch synthase genes have evolved from an ancestral gene in a different way or that the two genes are products of different ancestral genes that have converged during evolution. However, these two genes were closely located to each other on rice chromosome 6 at an approximate map distance of 5 centimorgans. The nucleotide sequence of the 5'-end region of the gene is unique because of the presence of some repetitive sequences.
一个编码某种形式可溶性淀粉合酶(SSS1)基因及其5'和3'侧翼区域的水稻(Oryza sativa L.)基因组克隆已被分离和测序。SSS1基因包含15个外显子,被14个内含子打断。SSS1基因的外显子/内含子结构与编码颗粒结合淀粉合酶的水稻蜡质基因不同,这表明SSS1基因和颗粒结合淀粉合酶基因是以不同方式从一个祖先基因进化而来,或者这两个基因是不同祖先基因的产物,在进化过程中发生了趋同。然而,这两个基因在水稻第6号染色体上彼此紧密相邻,大约相距5厘摩。由于存在一些重复序列,该基因5'端区域的核苷酸序列是独特的。