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豚鼠椭圆囊支持细胞中钙分布的变化

Changes in calcium distribution in the utricular supporting cells of the guinea pig.

作者信息

Mori N, Harada Y, Kasuga S, Oda Y, Hirakawa K, Yajin K

机构信息

Department of Oto-Rhino-Laryngology, Hiroshima University School of Medicine, Japan.

出版信息

Acta Otolaryngol Suppl. 1995;519:71-3. doi: 10.3109/00016489509121873.

DOI:10.3109/00016489509121873
PMID:7610895
Abstract

Changes in calcium distribution in cultured utricular supporting cells following streptomycin sulfate (SM) intoxication were investigated by means of an organ culture system using potassium pyroantimonate (PA) precipitation. PA precipitation showed that calcium ions are located in the otoconia and in the secretory granules of the supporting cells. Exposure of the supporting cells to SM increased the number of lysosomes and reduced the secretory granules in the cytoplasm. These lysosomes had many granules containing calcium ion. It is suggested that calcium secretion from the secretory granules was inhibited by SM and that this might lead to reduction of otoconia.

摘要

利用焦锑酸钾(PA)沉淀的器官培养系统,研究了硫酸链霉素(SM)中毒后培养的椭圆囊支持细胞中钙分布的变化。PA沉淀显示钙离子位于耳石和支持细胞的分泌颗粒中。支持细胞暴露于SM会增加溶酶体的数量,并减少细胞质中的分泌颗粒。这些溶酶体有许多含有钙离子的颗粒。提示SM抑制了分泌颗粒中的钙分泌,这可能导致耳石减少。

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