Oda Y
Department of Otolaryngology, Hiroshima University School of Medicine.
Nihon Jibiinkoka Gakkai Kaiho. 1993 Nov;96(11):1950-61. doi: 10.3950/jibiinkoka.96.1950.
Morphological changes in cultured utricular supporting cells following streptomycin sulfate (SM) intoxication were investigated using an organ culture system. Utricles of guinea pig were exposed to 30 and 3mg/ml of SM for 1-3 days in culture. The number of lysosomes in the supporting cells increased daily, and mitochondria, myeloid bodies, granules and vesicles were observed within the lysosomes. As these components accumulated in the lysosomes, the number of granules and vesicles in the cytoplasma decreased. Acid phosphatase (AcPase) activity also decreased. After 1-3 days culture with SM, the culture medium was changed to a medium without SM. After removal of SM, the Golgi apparati appeared more developed and AcPase activity was higher. At the same time, lysosomes were markedly decreased in number and the endoplasmic reticulum showed a gradual reproduction. These findings suggest close relationships among the Golgi apparatus, lysosomes, secretory granules and the endplasmic reticulum.
利用器官培养系统研究了硫酸链霉素（SM）中毒后培养的椭圆囊支持细胞的形态学变化。豚鼠椭圆囊在培养中暴露于30和3mg/ml的SM中1至3天。支持细胞中的溶酶体数量每日增加，并且在溶酶体内观察到线粒体、髓样小体、颗粒和囊泡。随着这些成分在溶酶体中积累，细胞质中的颗粒和囊泡数量减少。酸性磷酸酶（AcPase）活性也降低。用SM培养1至3天后，将培养基更换为不含SM的培养基。去除SM后，高尔基体显得更发达，AcPase活性更高。同时，溶酶体数量明显减少，内质网显示出逐渐再生。这些发现表明高尔基体、溶酶体、分泌颗粒和内质网之间存在密切关系。
