豚鼠椭圆囊支持细胞中耳石形成的过程。
The process of otoconia formation in guinea pig utricular supporting cells.
作者信息
Harada Y, Kasuga S, Mori N
机构信息
Hiroshima University, Japan.
出版信息
Acta Otolaryngol. 1998 Jan;118(1):74-9. doi: 10.1080/00016489850155161.
In order to clarify the mechanism of otoconia formation, the pathway of calcium transport in the utricular supporting cells was investigated. Potassium pyroantimonate (PA) precipitation, which indicates the localization of calcium ions, was seen not only in otoconia but also in supporting cell cytoplasm. In the latter, deposits of PA were detected in the secretory granules and endoplasmic reticulum (ER). These deposits were not present in cells pretreated with ethylene glycol-O,O,-bis (2-aminoethyl)-N,N,N',N'-tetra-acetic acid (EGTA). Exposure of the supporting cells to streptomycin sulfate (SM) increased the number of lysosomes. These lysosomes contained many small deposits of PA. The remaining granules and ER in cytoplasm also contained small deposits of PA. The findings suggest that otoconia are formed by the vestibular supporting cells in which calcium ions might be transported via ER-secretory granule-lysosome-cytoplasmic protrusion.
为了阐明耳石形成的机制，研究了椭圆囊支持细胞中钙的运输途径。焦锑酸钾（PA）沉淀表明钙离子的定位，不仅在耳石中可见，在支持细胞的细胞质中也可见。在后者中，在分泌颗粒和内质网（ER）中检测到PA沉积物。在用乙二醇 - O，O， - 双（2 - 氨基乙基） - N，N，N'，N' - 四乙酸（EGTA）预处理的细胞中不存在这些沉积物。将支持细胞暴露于硫酸链霉素（SM）会增加溶酶体的数量。这些溶酶体含有许多小的PA沉积物。细胞质中剩余的颗粒和内质网也含有小的PA沉积物。研究结果表明，耳石是由前庭支持细胞形成的，其中钙离子可能通过内质网 - 分泌颗粒 - 溶酶体 - 细胞质突起进行运输。
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