声学创伤和血管纹血流量后自由基的出现。

The emergence of free radicals after acoustic trauma and strial blood flow.

作者信息

Yamane H, Nakai Y, Takayama M, Konishi K, Iguchi H, Nakagawa T, Shibata S, Kato A, Sunami K, Kawakatsu C

机构信息

Department of Otolaryngology, Osaka City University Medical School, Japan.

出版信息

Acta Otolaryngol Suppl. 1995;519:87-92. doi: 10.3109/00016489509121877.

DOI:10.3109/00016489509121877

PMID:7610900

Abstract

The effect of acoustic trauma on cochlear strial circulation was investigated immunohistologically in the guinea pig. Kanamycin was used as a tracer of blood flow. Moreover, histochemical examinations were made to reveal the emergence of free radicals in the cochlea following acoustic trauma. At 5 min (5 min after intense sound exposure 120-125 dB SPL, 3 h) the blood flow in the stria vascularis was greatly diminished. At 2 h the strial blood flow started to recirculate and at 6 h it appeared to have returned to normal. Superoxide anion radicals (O2-) emerged along the luminal membrane of the marginal cells of the stria vascularis at 5 min. O2- disappeared at 30 min, but reappeared at 2 h. The cause of its emergence at 5 min was obscure. However, the strange phenomenon that O2- emerged again at 2 h seemed ascribable to the re-circulation of strial blood flow after sound exposure.

摘要

在豚鼠身上，采用免疫组织学方法研究了声创伤对耳蜗血管纹循环的影响。使用卡那霉素作为血流示踪剂。此外，还进行了组织化学检查，以揭示声创伤后耳蜗内自由基的出现情况。在5分钟时（在暴露于120 - 125 dB SPL的强声后5分钟，3小时），血管纹中的血流显著减少。在2小时时，血管纹血流开始重新循环，在6小时时似乎已恢复正常。超氧阴离子自由基（O2-）在5分钟时出现在血管纹边缘细胞的管腔膜沿线。O2-在30分钟时消失，但在2小时时再次出现。其在5分钟时出现的原因尚不清楚。然而，O2-在2小时时再次出现这一奇怪现象似乎归因于声暴露后血管纹血流的重新循环。