Rollin P E, Bowen M D, Kariwa H, Saluzzo J F, Guerard S, Flechaire A, Coudrier D, Sureau P, Peters C J, Nichol S T
Centre National de Reference pour les Fievres Hemorragiques Virales, Institut Pasteur, Paris.
Am J Trop Med Hyg. 1995 Jun;52(6):577-8. doi: 10.4269/ajtmh.1995.52.577.
Puumala (PUU) virus (Bunyaviridae: Hantavirus), the etiologic agent of nephropathia epidemica (NE), the mid form of hemorrhagic fever with renal syndrome, is enzootic in Europe and has been known to occur in France since 1983. We report the first isolation of PUU virus in France and western Europe from a case of NE acquired in France. The virus was isolated from a serum collected in the acute phase of the clinical course by successive blind passages in Vero E6 cells. Serologic typing using monoclonal antibodies confirmed the identity of the virus as PUU. The sequence of an 832-nucleotide fragment of the virus medium RNA segment obtained by the polymerase chain reaction (PCR) also classified it as a PUU virus. The sequence of this isolate from a human case in France is closely related to the sequence of a PUU virus obtained by the PCR from a German patient.
普马拉（PUU）病毒（布尼亚病毒科：汉坦病毒）是肾综合征出血热（HFRS）中型肾综合征出血热（NE）的病原体，该病毒在欧洲呈地方性流行，自1983年以来在法国也有病例报道。我们报告了法国和西欧首例从法国获得的NE病例中分离出PUU病毒。该病毒是从临床病程急性期采集的血清中，通过在Vero E6细胞中连续盲传分离得到的。使用单克隆抗体进行血清学分型证实该病毒为PUU病毒。通过聚合酶链反应（PCR）获得的病毒中RNA片段的832个核苷酸序列也将其归类为PUU病毒。此法国人类病例分离株的序列与通过PCR从一名德国患者获得的PUU病毒序列密切相关。
