Hörling J, Lundkvist A, Jaarola M, Plyusnin A, Tegelström H, Persson K, Lehväslaiho H, Hörnfeldt B, Vaheri A, Niklasson B
Swedish Institute for Infectious Disease Control, Stockholm.
J Gen Virol. 1996 Oct;77 ( Pt 10):2555-62. doi: 10.1099/0022-1317-77-10-2555.
Small mammals trapped in Sweden were analysed for specific antibody responses against three hantavirus serotypes and for the presence of viral antigen. To determine the genetic identity of viral RNA in lungs of seropositive bank voles (Clethrionomys glareolus), polymerase chain reactions and subsequent partial sequencing of both the M and S segments were employed. The sequences obtained were all identified as Puumala (PUU) virus, with a high degree of heterogeneity between the different geographical localities. Alignment of nucleotide and deduced amino acid sequences, together with phylogenetic analysis, showed that PUU viruses circulating in central Sweden were distinct from those in the northern region. The localization of the two distinct PUU virus genotypes was shown to correlate with the postglacial recolonization of Sweden by bank voles.
对在瑞典捕获的小型哺乳动物进行分析，检测其针对三种汉坦病毒血清型的特异性抗体反应以及病毒抗原的存在情况。为了确定血清阳性棕背䶄（Clethrionomys glareolus）肺中病毒RNA的基因身份，采用了聚合酶链反应以及随后对M和S片段进行的部分测序。所获得的序列均被鉴定为普马拉（PUU）病毒，不同地理区域之间存在高度异质性。核苷酸和推导氨基酸序列的比对以及系统发育分析表明，瑞典中部流行的PUU病毒与北部地区的不同。两种不同的PUU病毒基因型的分布与棕背䶄在瑞典冰期后重新定殖的情况相关。
