一株瑞典普马拉病毒分离株M和S基因组片段的核苷酸及推导的氨基酸序列

Nucleotide and deduced amino acid sequences of the M and S genome segments of a Swedish Puumala virus isolate.

作者信息

Hörling J, Cheng Y, Plyusnin A, Persson K, Lehväslaiho H, Vaheri A, Niklasson B, Lundkvist A

机构信息

Department of Defense Microbiology, Swedish Institute for Infectious Disease Control, Stockholm.

出版信息

Virus Res. 1995 Dec;39(2-3):321-30. doi: 10.1016/0168-1702(95)00081-x.

DOI:10.1016/0168-1702(95)00081-x

PMID:8837894

Abstract

The Swedish Puumala (PUU) virus strain Vindeln 83-L20, isolated from a bank vole trapped in 1983 near Vindeln, Västerbotten county, Sweden, was characterized by nucleotide sequence analysis. The coding region of the M segment was determined by PCR followed by direct sequencing and the entire S segment was characterized by cloning and nucleotide sequence analysis. The genomic organization was found to be very similar to that of other PUU virus strains regarding open reading frames, polypeptide sizes and potential glycosylation sites. According to phylogenetic analysis 83-L20 was found to represent a new lineage within the Puumala virus serotype in the Hantavirus genus. The M segment sequence of 83-L20 was found to be more closely related to the Finnish PUU virus strains than to strains from Central Europe or from Russia. The evolutionary origin of the S segment was not as clearly resolved since the branching points of all PUU virus strains in the phylogenetic tree were nearly the same.

摘要

对从1983年在瑞典韦斯特博滕省文德尔恩附近捕获的一只棕背䶄身上分离出的瑞典普马拉（PUU）病毒株Vindeln 83-L20进行了核苷酸序列分析。通过聚合酶链反应（PCR）随后直接测序确定了M片段的编码区，并通过克隆和核苷酸序列分析对整个S片段进行了特征鉴定。在开放阅读框、多肽大小和潜在糖基化位点方面，发现该病毒的基因组结构与其他PUU病毒株非常相似。根据系统发育分析，发现83-L20代表汉坦病毒属普马拉病毒血清型内的一个新谱系。发现83-L20的M片段序列与芬兰的PUU病毒株的关系比与来自中欧或俄罗斯的病毒株更为密切。由于系统发育树中所有PUU病毒株的分支点几乎相同，S片段的进化起源没有得到同样清晰的解析。