Leon-Monzon M E, Illa I, Dalakas M C
Medical Neurology Branch, National Institute of Neurological Diseases and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA.
Ann N Y Acad Sci. 1995 May 25;753:48-57. doi: 10.1111/j.1749-6632.1995.tb27530.x.
Because a prerequisite for infection of a cell with the poliovirus is the presence of poliovirus receptor (PVR), we examined its tissue localization in the human muscle, spinal cord, and muscle cultures using a specific monoclonal antibody against PVR in immunocytochemical studies on serial sections. We found weak expression of PVR in the motor neurons but not the axons. In normal muscle, PVR was expressed at the end plate as confirmed by immunolocalization in serial sections with alpha-bungarotoxin. In neurogenic conditions and in myopathies, PVR was found in occasional denervated muscle fibers and in several regenerating ones. Human myotubes expressed PVR and were susceptible to the poliovirus infection. We conclude that PVR is present at the motor end-plate that can serve as one of the routes of entry of the virus to the motor neurons. The presence of PVR in the regenerating muscle fibers is in accord with clinical observations that muscle injuries can predispose patients to paralytic poliomyelitis.
由于脊髓灰质炎病毒感染细胞的一个前提条件是存在脊髓灰质炎病毒受体（PVR），我们在连续切片的免疫细胞化学研究中，使用针对PVR的特异性单克隆抗体，检测了其在人类肌肉、脊髓和肌肉培养物中的组织定位。我们发现PVR在运动神经元中有微弱表达，但在轴突中未表达。在正常肌肉中，通过用α-银环蛇毒素对连续切片进行免疫定位证实，PVR在终板处表达。在神经源性疾病和肌病中，在偶尔失神经支配的肌纤维和一些再生肌纤维中发现了PVR。人肌管表达PVR并易受脊髓灰质炎病毒感染。我们得出结论，PVR存在于运动终板，可作为病毒进入运动神经元的途径之一。PVR在再生肌纤维中的存在与肌肉损伤可使患者易患麻痹性脊髓灰质炎的临床观察结果一致。
