Kimura O, Hisamitsu K, Sugamura K, Nakamura S, Kurayoshi K, Makino M, Kaibara N
First Dept. of Surgery, Faculty of Medicine, Tottori University.
Gan To Kagaku Ryoho. 1995 Jun;22 Suppl 2:134-9.
To investigate the possible relation between p53 protein, DNA content and liver metastasis of colorectal cancer, overexpression of p53 and DNA content were measured by flowcytometer in 113 primary lesions, which included 34 cases with simultaneous liver metastasis and 79 cases with curative resection, and 25 metastatic lesions of the liver. Overexpression of p53 and DNA aneuploidy were found in 44 (38.9%) and 77 (68.1%) of 113 primary lesions, respectively. However, p53 protein and DNA aneuploidy were unrelated to the clinicopathological findings, such as liver metastasis, venous invasion and lymph node metastasis. Comparing the overexpression of p53 protein between primary and metastatic lesions, p53 protein was recognized in 18 (72.0%) of 25 metastatic lesions of the liver. Incidence of p53 protein was significantly higher in metastatic lesions of the liver than in primary lesions (p < 0.01). On the other hand, p53 protein was found in 27 (60.0%) of 45 diploid lesions and in 35 (37.6%) of 93 aneuploid lesions. There was a significant difference in p53 protein between diploid and aneuploid tumors (p < 0.05). These results suggest that p53 protein may not correlate with the occurrence of liver metastasis and might be produced in the metastatic lesion of the liver after metastasis.
为了研究p53蛋白、DNA含量与结直肠癌肝转移之间的可能关系，采用流式细胞仪检测了113例原发性病灶（其中34例同时伴有肝转移，79例接受根治性切除）及25例肝脏转移病灶中的p53过表达情况和DNA含量。在113例原发性病灶中，分别有44例（38.9%）出现p53过表达，77例（68.1%）出现DNA非整倍体。然而，p53蛋白和DNA非整倍体与临床病理表现，如肝转移、静脉侵犯和淋巴结转移无关。比较原发性病灶和转移病灶中p53蛋白的过表达情况，在25例肝脏转移病灶中有18例（72.0%）检测到p53蛋白。肝脏转移病灶中p53蛋白的发生率显著高于原发性病灶（p < 0.01）。另一方面，在45例二倍体病灶中有27例（60.0%）检测到p53蛋白，在93例非整倍体病灶中有35例（37.6%）检测到p53蛋白。二倍体肿瘤和非整倍体肿瘤之间的p53蛋白存在显著差异（p < 0.05）。这些结果表明，p53蛋白可能与肝转移的发生无关，可能是在转移后在肝脏转移病灶中产生的。
