肌肉纤维赤道X射线衍射图谱分析：影响强度的因素
Analysis of equatorial x-ray diffraction patterns from muscle fibers: factors that affect the intensities.
作者信息
Malinchik S, Yu L C
机构信息
Laboratory of Physical Biology, National Institute of Arthritis, Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
出版信息
Biophys J. 1995 May;68(5):2023-31. doi: 10.1016/S0006-3495(95)80379-6.
Abstract
Previously we have shown that cross-bridge attachment to actin and the radial position of the myosin heads surrounding the thick filament backbone affect the equatorial x-ray diffraction intensities in different ways (Yu, 1989). In the present study, other factors frequently encountered experimentally are analyzed by a simple model of the filament lattice. It is shown that the ordering/disordering of filaments, lattice spacing changes, the azimuthal redistributions of cross-bridges, and variations in the ordered/disordered population of cross-bridges surrounding the thick filaments can distinctly affect the equatorial intensities. Consideration of Fourier transforms of individual components of the unit cell can provide qualitative explanations for the equatorial intensity changes. Criteria are suggested that can be used to distinguish the influence of some factors from others.
摘要
此前我们已经表明，横桥与肌动蛋白的附着以及围绕粗肌丝主干的肌球蛋白头部的径向位置，会以不同方式影响赤道X射线衍射强度（Yu，1989）。在本研究中，通过细丝晶格的简单模型分析了实验中经常遇到的其他因素。结果表明，细丝的有序/无序、晶格间距变化、横桥的方位角重新分布以及围绕粗丝的横桥有序/无序群体的变化，都能明显影响赤道强度。对晶胞各个组分的傅里叶变换进行考量，可以为赤道强度变化提供定性解释。提出了一些标准，可用于区分某些因素的影响。
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本文引用的文献
1
An X-ray diffraction study of frog skeletal muscle during shortening near the maximum velocity.对青蛙骨骼肌在接近最大速度缩短过程中的X射线衍射研究。
J Mol Biol. 1993 Jun 5;231(3):668-77. doi: 10.1006/jmbi.1993.1318.
2
Structural changes in the actomyosin cross-bridges associated with force generation.与力产生相关的肌动球蛋白横桥的结构变化。
Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5252-6. doi: 10.1073/pnas.90.11.5252.
3
Three-dimensional structure of myosin subfragment-1: a molecular motor.肌球蛋白亚片段-1的三维结构：一种分子马达。
Science. 1993 Jul 2;261(5117):50-8. doi: 10.1126/science.8316857.
4
Changes in the X-ray reflections from contracting muscle during rapid mechanical transients and their structural implications.快速机械瞬变期间收缩肌肉的X射线反射变化及其结构意义。
J Mol Biol. 1983 Sep 15;169(2):469-506. doi: 10.1016/s0022-2836(83)80062-x.
5
X-ray diffraction evidence for cross-bridge formation in relaxed muscle fibers at various ionic strengths.在不同离子强度下，松弛肌纤维中横桥形成的X射线衍射证据。
Biophys J. 1984 Sep;46(3):299-306. doi: 10.1016/S0006-3495(84)84026-6.
6
Structural difference between resting and rigor muscle; evidence from intensity changes in the lowangle equatorial x-ray diagram.静息肌肉与僵直肌肉之间的结构差异；来自低角度赤道X射线图强度变化的证据。
J Mol Biol. 1968 Nov 14;37(3):507-20. doi: 10.1016/0022-2836(68)90118-6.
7
Structure of insect fibrillar flight muscle in the presence and absence of ATP.有ATP和无ATP情况下昆虫纤维状飞行肌肉的结构
J Mol Biol. 1972 Sep 14;70(1):85-104. doi: 10.1016/0022-2836(72)90165-9.
8
Equatorial x-ray diffraction from single skinned rabbit psoas fibers at various degrees of activation. Changes in intensities and lattice spacing.不同激活程度下单层兔腰大肌纤维的赤道X射线衍射。强度和晶格间距的变化。
Biophys J. 1985 Nov;48(5):829-34. doi: 10.1016/S0006-3495(85)83841-8.
9
Distribution of mass in relaxed frog skeletal muscle and its redistribution upon activation.松弛状态下青蛙骨骼肌的质量分布及其激活后的重新分布。
Biophys J. 1985 Mar;47(3):311-21. doi: 10.1016/S0006-3495(85)83921-7.
10
X-ray study of myosin heads in contracting frog skeletal muscle.收缩的青蛙骨骼肌中肌球蛋白头部的X射线研究。
J Mol Biol. 1987 Apr 20;194(4):595-600. doi: 10.1016/0022-2836(87)90236-1.
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