香菇中第二种5'-核苷酸形成核酸酶的纯化与特性分析

Purification and characterization of the 2nd 5'-nucleotide-forming nuclease from Lentinus edodes.

作者信息

Kobayashi H, Inokuchi N, Koyama T, Tomita M, Irie M

机构信息

Department of Microbiology, College of Pharmacy, Nihon University, Chiba.

出版信息

Biosci Biotechnol Biochem. 1995 Jun;59(6):1169-71. doi: 10.1271/bbb.59.1169.

DOI:10.1271/bbb.59.1169

PMID:7613009

Abstract

The 2nd endonuclease (nuclease Le3) which hydrolyzes both RNA and heat denatured DNA to 5'-nucleotides was purified from the fruit bodies of Lentinus edodes to a homogeneous state by SDS PAGE. The nuclease was different from the nuclease Le1 previously characterized [H. Shimada et al. Chem. Pharm. Bull., 39, 2633-2637 (1991)] in molecular weight, optimal pH and N-terminal amino acid sequence. The N-terminal amino acid sequence of nuclease Le3 analyzed up to the 20th residue showed that 50% of the amino acid residues are identical to nuclease Le1.

摘要

从香菇子实体中纯化出第二种核酸内切酶（核酸酶Le3），它能将RNA和热变性DNA水解为5'-核苷酸，并通过SDS-PAGE达到了均一状态。该核酸酶在分子量、最适pH值和N端氨基酸序列方面与先前鉴定的核酸酶Le1[H. Shimada等人，《化学与药学通报》，39，2633 - 2637（1991）]不同。对核酸酶Le3的N端氨基酸序列分析至第20个残基，结果显示50%的氨基酸残基与核酸酶Le1相同。