Beier D R, Dushkin H, Telle T
Genetics Division, Brigham and Women's Hospital, Boston, Massachusetts 02115, USA.
Mamm Genome. 1995 Apr;6(4):269-72. doi: 10.1007/BF00352414.
We have determined the order of a number of SSR and SSC polymorphic markers that map to distal mouse Chromosome (Chr) 4 and have used analysis of these markers in backcrosses designed to test the localization of the curly-tail (ct) mutation. We have confirmed that ct maps to this region, close to the locus D4Mit69. Our results also support the hypothesis that ct is a semidominant, rather than a recessive, mutation, since we have identified abnormal-tailed mice that are likely to be heterozygous at the ct locus. Finally, we examined Pax7 as a candidate gene for the ct mutation and found no evidence of protein sequence differences in ct compared with wild-type mice.
我们已经确定了多个映射到小鼠4号染色体远端的简单序列重复（SSR）和简单序列簇（SSC）多态性标记的顺序，并在用于测试卷尾（ct）突变定位的回交实验中对这些标记进行了分析。我们已经证实ct定位于该区域，靠近D4Mit69位点。我们的结果还支持这样的假设，即ct是一种半显性而非隐性突变，因为我们已经鉴定出在ct位点可能为杂合子的异常尾巴小鼠。最后，我们将Pax7作为ct突变的候选基因进行了检测，未发现与野生型小鼠相比ct在蛋白质序列上存在差异的证据。
