Vrtala S, Grote M, Ferreira F, Susani M, Stocker B, Kraft D, Valenta R
Institute of General and Experimental Pathology, AKH, University of Vienna, Austria.
Int Arch Allergy Immunol. 1995 May-Jun;107(1-3):290-4. doi: 10.1159/000237005.
Recombinant tree pollen allergens (recombinant Bet v I and recombinant birch profilin, Bet v II) were purified and used to immunize BALB/c and B6D2F1 mice with Al(OH)3 to elicit a specific IgE response. Serum from immunized mice was then used to detect immunoblotted natural tree pollen allergens. The onset of the humoral immune response was monitored using antimouse IgE, IgG1, IgG2a/b, IgG3 and IgA. In both strains, a specific and long-lasting IgE response could be elicited with both recombinant allergens. Mice immunized continuously with recombinant Bet v I + Al(OH)3 showed a significant decrease of specific IgE antibodies indicating that continuous application of allergens can reduce specific IgE responses. The possibility of inducing a different type of immune responses is indicated by the fact that mice fed with Bet v I expressed in apathogenic Salmonella strains showed a Th1 immune response to Bet v I accompanied by specific IgG2a/b without detectable IgG1 or IgE. Recombinant allergens can hence be used to decrease or even modulate specific IgE responses in vivo.
重组树花粉过敏原（重组Bet v I和重组桦树肌动蛋白结合蛋白，Bet v II）经纯化后，与氢氧化铝一起用于免疫BALB/c和B6D2F1小鼠，以引发特异性IgE反应。然后用免疫小鼠的血清检测经免疫印迹法处理的天然树花粉过敏原。使用抗小鼠IgE、IgG1、IgG2a/b、IgG3和IgA监测体液免疫反应的发生。在这两种品系中，两种重组过敏原均可引发特异性且持久的IgE反应。用重组Bet v I +氢氧化铝持续免疫的小鼠显示特异性IgE抗体显著减少，表明持续应用过敏原可降低特异性IgE反应。用无致病性沙门氏菌菌株表达的Bet v I喂养的小鼠对Bet v I表现出Th1免疫反应，并伴有特异性IgG2a/b，而未检测到IgG1或IgE，这一事实表明存在诱导不同类型免疫反应的可能性。因此，重组过敏原可用于在体内降低甚至调节特异性IgE反应。
