Vrtala S, Mayer P, Ferreira F, Susani M, Sehon A H, Kraft D, Valenta R
Institute of General and Experimental Pathology, AKH, University of Vienna, Austria.
J Allergy Clin Immunol. 1996 Nov;98(5 Pt 1):913-21. doi: 10.1016/s0091-6749(96)80007-8.
Serologic measurements with recombinant birch pollen allergens, rBet v 1 and rBet v 2 (birch profilin), have shown that more than 95% of patients allergic to tree pollen mount high levels of IgE against rBet v 1, whereas only approximately 10% of the patients display rather low levels of IgE against rBet v 2.
In this study an attempt was made to determine whether the different allergenicity of the major birch pollen allergen, rBet v 1, and a minor birch pollen allergen, rBet v 2, might be related to a different immunogenicity of the proteins as evaluated in experimental animal systems (mice and rhesus monkeys).
Purified recombinant allergens were injected into mice and rhesus monkeys with aluminum hydroxide as adjuvant for elicitation of specific IgE responses. Antibody responses to the allergens were detected by immunoblotting, and time courses of immune responses were measured by ELISA.
In both animal models more than the 10-fold dose of rBet v 2 was required to induce IgE antibodies, and even then, the amount of specific IgE antibodies elicited with rBet v 1 was substantially higher than that induced by rBet v 2. It was noted that rBet v 2 formed stable polymers through disulfide bonds.
In two different animal models (mice and rhesus monkeys) the major birch pollen allergen, rBet v 1, induced substantially higher levels of IgE than rBet v 2. A reduced allergenicity of Bet v 2 caused by polymer formation would be in agreement with previous studies indicating reduced allergenicity of proteins on chemical polymerization.
使用重组桦树花粉过敏原rBet v 1和rBet v 2（桦树肌动蛋白结合蛋白）进行的血清学检测表明，超过95%的对树花粉过敏的患者针对rBet v 1产生高水平的IgE，而只有约10%的患者针对rBet v 2表现出相当低水平的IgE。
在本研究中，试图确定主要桦树花粉过敏原rBet v 1和次要桦树花粉过敏原rBet v 2的不同致敏性是否可能与在实验动物系统（小鼠和恒河猴）中评估的蛋白质的不同免疫原性有关。
将纯化的重组过敏原与氢氧化铝作为佐剂注射到小鼠和恒河猴体内，以引发特异性IgE反应。通过免疫印迹检测对过敏原的抗体反应，并通过ELISA测量免疫反应的时间进程。
在两种动物模型中，诱导IgE抗体都需要超过10倍剂量的rBet v 2，即便如此，rBet v 1引发的特异性IgE抗体量仍显著高于rBet v 2引发的量。值得注意的是，rBet v 2通过二硫键形成稳定的聚合物。
在两种不同的动物模型（小鼠和恒河猴）中，主要桦树花粉过敏原rBet v 1诱导的IgE水平显著高于rBet v 2。由聚合物形成导致的Bet v 2致敏性降低与先前表明化学聚合后蛋白质致敏性降低的研究结果一致。
