Hammond G L, Leinonen P, Vihko R
Clin Chem. 1979 Jan;25(1):129-31.
We describe a discrete automated radioimmunoassay system for determining choriomammotropin (placental lactogen) in human serum. With the present system it can be measured in as many as 37 unknown sera (50 muL) and three quality-control sera, in duplicate, within 1.5 h. The time required for sample preparation, incubation (15 min), and separation of free and bound radioactivities (a 150 mL/L polyethylene glycol solution is superior to a twofold volume of absolute ethanol) is less than 45 min. The remaining time required is for counting and data processing. Intra-assay precision is 4.6% (CV). The modular approach endows the instrumentation with much flexibility, and consequently is suitable for automation of a wide range of assay protocols.
我们描述了一种用于测定人血清中绒毛膜促乳腺素（胎盘催乳素）的离散自动放射免疫分析系统。使用本系统，可在1.5小时内对多达37份未知血清（50μL）和3份质量控制血清进行双份测定。样品制备、孵育（15分钟）以及游离和结合放射性物质的分离（150 mL/L聚乙二醇溶液优于两倍体积的无水乙醇）所需时间少于45分钟。其余所需时间用于计数和数据处理。批内精密度为4.6%（CV）。模块化方法使该仪器具有很大的灵活性，因此适用于广泛的分析方案自动化。
