聚乙二醇在人胎盘催乳素放射免疫测定中的应用。
Use of polyethylene glycol in radioimmunoassay of human placental lactogen.
作者信息
Ermshar C L, Gusseck D J
出版信息
Clin Chem. 1978 Oct;24(10):1767-0.
PMID:699287
Abstract
We describe a rapid radioimmunoassay for human placental lactogen in biological fluids, with use of polyethylene glycol to separate free from antibody-bound placental lactogen. The standard curve obtained, easily fitted to a logit-log transformation, is useful over a concentration range of 6 to 400 ug/liter. Within-assay variability is 1.97%, between-assay variability 2.20%. The relation is linear when lactogen added is plotted against that accounted for analytically, the actual recovery being 100.26%.
摘要
我们描述了一种用于生物体液中人胎盘催乳素的快速放射免疫测定法，该方法使用聚乙二醇来分离游离的和与抗体结合的胎盘催乳素。所获得的标准曲线易于拟合为logit-对数转换，在6至400微克/升的浓度范围内有效。批内变异系数为1.97%，批间变异系数为2.20%。当将添加的催乳素与分析测定的催乳素作图时，两者关系呈线性，实际回收率为100.26%。
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