Price C P, Spencer K
Clin Chem. 1981 Jun;27(6):882-7.
The sensitivity of kinetic immunoturbidimetry as a method for the measurement of specific proteins has been shown previously to be equivalent to 1 mg of analyte per liter. We confirm this in the method for human choriomammotropin described here. A relatively large sample volume is required, which results in other components of serum influencing the immunoprecipitin reaction. The technique is shown to be precise, and results compare well with those by a commonly available radioimmunoassay. The method performs well, as judged from results obtained for assayed quality control materials and in an external quality assurance program. The method is rapid (instrument assay time 120 s per sample), automated, and economical for routine use.
动力学免疫比浊法作为一种测定特定蛋白质的方法，其灵敏度先前已被证明相当于每升1毫克分析物。我们在此处描述的人绒毛膜促乳腺素测定方法中证实了这一点。该方法需要相对较大的样本量，这会导致血清中的其他成分影响免疫沉淀反应。结果表明该技术具有精确性，并且与常用的放射免疫分析法所得结果具有良好的可比性。根据测定质量控制材料以及外部质量保证计划所获得的结果判断，该方法性能良好。该方法快速（每个样本的仪器测定时间为120秒）、自动化，且适用于常规使用，经济实惠。
