Midha K K, Loo J C, Hubbard J W, Rowe M L, McGilveray I J
Clin Chem. 1979 Jan;25(1):166-8.
A radioimmunoassay for chlorpromazine in plasma is described. The antiserum was obtained by immunizing rabbits with a conjugate of bovine serum albumin and N-(2-carboxyethyl)desmethylchlorpromazine. It is specific for chlorpromazine and its minor active metabolite, N-desmethylchlorpromazine. Other known active or inactive chlorpromazine metabolites and other psychotropic drugs tested do not cross react with the antiserum. Less than 34 pg of the drug can be detected in 200 muL of plasma. As many as 100 samples can be processed in a day by one technician. Concentrations of chlorpromazine can be measured in 200-muL samples of plasma collected as late as 48 h after a single oral 25-mg dose.
本文描述了一种血浆中氯丙嗪的放射免疫分析方法。抗血清是通过用牛血清白蛋白与N -（2 - 羧乙基）去甲氯丙嗪的偶联物免疫兔子获得的。它对氯丙嗪及其次要活性代谢物N - 去甲氯丙嗪具有特异性。所测试的其他已知活性或非活性氯丙嗪代谢物及其他精神药物与抗血清不发生交叉反应。在200μL血浆中可检测到少于34pg的该药物。一名技术人员一天可处理多达100个样本。单次口服25mg剂量后最晚在48小时采集的200μL血浆样本中可测量氯丙嗪的浓度。
