Aguilar-Cordova E, Chinen J, Donehower L A, Harper J W, Rice A P, Butel J S, Belmont J W
Department of Pediatrics, Baylor College of Medicine, Houston, TX 77030, USA.
Gene Ther. 1995 May;2(3):181-6.
A double transdominant fusion gene (trev) designed to inhibit two essential HIV functions simultaneously was constructed by linking tat and rev transdominant mutants. Trev independently inhibited both Tat and Rev functions, localized within the nucleus and cells transfected with trev showed a stable inhibition of HIV-1-mediated cytopathicity. A retroviral vector of trev was made and shown also to confer protection from HIV cytopathic effects. Simultaneous inhibition of two essential viral genes presents significant advantages for potential gene therapy treatment of HIV infection over conventional single effect molecules.
通过连接tat和rev反式显性突变体构建了一个旨在同时抑制HIV两种基本功能的双反式显性融合基因（trev）。Trev能独立抑制Tat和Rev功能，定位于细胞核内，转染trev的细胞对HIV-1介导的细胞病变具有稳定的抑制作用。制备了trev的逆转录病毒载体，其也显示出能抵御HIV细胞病变效应。与传统的单效应分子相比，同时抑制两种基本病毒基因在潜在的HIV感染基因治疗中具有显著优势。
