Negendank W G, Padavic-Shaller K A, Li C W, Murphy-Boesch J, Stoyanova R, Krigel R L, Schilder R J, Smith M R, Brown T R
Department of Nuclear Magnetic Resonance and Medical Spectroscopy, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA.
Cancer Res. 1995 Aug 1;55(15):3286-94.
Development of biological and clinical uses of in vivo 31P magnetic resonance spectroscopy has been hampered by poor anatomic localization of spectra and poor resolution of overlapping signals within phosphomonoester and phosphodiester regions of the spectrum. We applied 1H-decoupling and nuclear Overhauser enhancement to improve resolution of 31P magnetic resonance spectra accurately localized to 21 non-Hodgkin's lymphomas (NHL) by using three-dimensional chemical shift imaging. All 21 spectra had large phosphomonoester signals (26% of total phosphorus) that contained high amounts of phosphoethanolamine relative to phosphocholine. There were no signals from glycerophosphoethanolamine or glycerophosphocholine but only a broad signal from membrane phospholipids in the phosphodiester region (20% of phosphorus). Prominent nucleoside triphosphates (47% of phosphorus) and low inorganic phosphate (7% of phosphorus) indicate well-perfused tissue with viable cells. Mean intracellular pH was 7.23. These characteristics were similar in all grades and stages of NHL. By analogy with recently reported studies in cell lines in vitro, we hypothesize that the pattern of phospholipid metabolites observed in NHL in vivo is partly a manifestation of sustained activation of phospholipase C or D. The techniques we implemented permitted us to obtain more information about in vivo metabolism of NHL than has heretofore been available. This information is important for the establishment of appropriate experimental models and provides a basis from which to examine potential clinical uses of 31P magnetic resonance spectroscopy.
体内31P磁共振波谱学在生物学和临床应用方面的发展受到了阻碍，原因在于波谱的解剖定位不佳以及在波谱的磷酸单酯和磷酸二酯区域内重叠信号的分辨率较差。我们应用1H去耦和核Overhauser增强技术，通过三维化学位移成像来提高31P磁共振波谱的分辨率，并将其准确地定位到21例非霍奇金淋巴瘤（NHL）。所有21个波谱都有较大的磷酸单酯信号（占总磷的26%），相对于磷酸胆碱而言，该信号中含有大量的磷酸乙醇胺。没有来自甘油磷酸乙醇胺或甘油磷酸胆碱的信号，在磷酸二酯区域仅存在来自膜磷脂的一个宽信号（占磷的20%）。显著的核苷三磷酸（占磷的47%）和低无机磷酸盐（占磷的7%）表明组织灌注良好且细胞存活。平均细胞内pH值为7.23。这些特征在NHL的所有分级和分期中都是相似的。通过与最近在体外细胞系中报道的研究进行类比，我们推测在体内NHL中观察到的磷脂代谢物模式部分是磷脂酶C或D持续激活的一种表现。我们实施的技术使我们能够获得比以往更多的关于NHL体内代谢的信息。这些信息对于建立合适的实验模型很重要，并为研究31P磁共振波谱的潜在临床应用提供了基础。
