Li C W, Kuesel A C, Padavic-Shaller K A, Murphy-Boesch J, Eisenberg B L, Schmidt R G, von Roemeling R W, Patchefsky A S, Brown T R, Negendank W G
Department of Nuclear Magnetic Resonance and Medical Spectroscopy, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA.
Cancer Res. 1996 Jul 1;56(13):2964-72.
We applied 1H-decoupling and nuclear Overhauser enhancement to obtain well-resolved 31P magnetic resonance spectra accurately localized to 20 soft tissue sarcomas in vivo, using three-dimensional chemical shift imaging. Fifteen spectra had large phosphomonoester signals (21% of total phosphorus) that contained high amounts of phosphoethanolamine (compared to those of phosphocholine) but no signals from glycerophosphoethanolamine, and glycerophosphocholine was detected in only four cases. Prominent nucleoside triphosphates (52% of phosphorus) and low inorganic phosphate (10% of phosphorus) indicated that a large fraction of these 15 sarcomas contained viable cells, and this impression was confirmed histologically in 13 of the sarcomas. High-resolution in vitro 31P spectra of extracts of surgical specimens of four of the sarcomas studied in vivo and six additional sarcomas confirmed the in vivo assignments of metabolites and revealed considerable inter- and intratumoral variations of metabolite concentrations associated with histological variations in the relative amounts of cells and of matrix materials or spontaneous necrosis. Seven sarcomas, all high grade with pleomorphic or round cells rather than spindle cells, contained an unidentified phosphodiester signal in vivo; its absence in the extract spectra indicates that it may be from an abnormally mobile membrane component. We have documented a means to obtain new information about in vivo metabolism in human sarcomas and to provide a basis on which to examine the uses of 31P magnetic resonance spectroscopy in the clinical management of sarcomas.
我们应用¹H去耦和核Overhauser增强技术，通过三维化学位移成像，在体内准确地定位到20例软组织肉瘤，从而获得分辨率良好的³¹P磁共振波谱。15个波谱具有较大的磷酸单酯信号（占总磷的21%），其中含有大量的磷酸乙醇胺（与磷酸胆碱相比），但未检测到甘油磷酸乙醇胺的信号，仅在4例中检测到甘油磷酸胆碱。显著的核苷三磷酸（占磷的52%）和低无机磷酸盐（占磷的10%）表明，这15个肉瘤中的很大一部分含有活细胞，并且在13个肉瘤中通过组织学证实了这一印象。对体内研究的4个肉瘤以及另外6个肉瘤的手术标本提取物进行的高分辨率体外³¹P波谱分析，证实了体内代谢物的归属，并揭示了代谢物浓度在肿瘤间和肿瘤内存在相当大的差异，这些差异与细胞和基质材料的相对数量的组织学变化或自发坏死有关。7个肉瘤，均为高级别，具有多形性或圆形细胞而非梭形细胞，在体内含有一个未鉴定的磷酸二酯信号；其在提取物波谱中的缺失表明它可能来自异常移动的膜成分。我们记录了一种方法，可获取有关人类肉瘤体内代谢的新信息，并为研究³¹P磁共振波谱在肉瘤临床管理中的应用提供依据。
