Erf G F, Trejo-Skalli A V, Smyth J R
Department of Biological Sciences, Smith College, Northampton, Massachusetts 01063, USA.
Clin Immunol Immunopathol. 1995 Aug;76(2):120-6. doi: 10.1006/clin.1995.1105.
Smyth line (SL) chickens exhibit a spontaneous, autoimmune, depigmentation (SL vitiligo, SLV) in regenerating feather tissue due to the death of pigment cells (melanocytes). It was the purpose of this study to characterize mononuclear leukocytes which infiltrate the feather as SLV develops. Frozen sections of regenerating features from SL and control chickens were labeled with a panel of mAbs specific for chicken mononuclear leukocytes. SL chickens were found to have significantly greater numbers of T cells in the feather pulp prior to, and throughout, the development of SLV. Initially, both SL and control chickens had a CD4/CD8 ratio near 1.0 in their feather pulp. Following the onset of SLV, the CD4/CD8 ratio in SL chickens dropped below 0.4. Throughout the development of SLV, SL chickens had greater proportions of T cells with alpha/beta T cell receptors than controls. Histological evidence strongly supports an important role for T cells in the pathology of SLV.
斯迈思系（SL）鸡在再生羽毛组织中表现出一种由于色素细胞（黑素细胞）死亡导致的自发性自身免疫性色素脱失（SL白癜风，SLV）。本研究的目的是对随着SLV发展而浸润羽毛的单核白细胞进行特征描述。用一组针对鸡单核白细胞的单克隆抗体标记来自SL鸡和对照鸡的再生羽毛的冰冻切片。发现在SLV发生之前及整个过程中，SL鸡羽毛髓质中的T细胞数量显著更多。最初，SL鸡和对照鸡羽毛髓质中的CD4/CD8比值均接近1.0。SLV发病后，SL鸡的CD4/CD8比值降至0.4以下。在SLV发展的整个过程中，SL鸡具有α/βT细胞受体的T细胞比例高于对照鸡。组织学证据有力地支持了T细胞在SLV病理学中起重要作用。
