Erf G F, Smyth J R
Department of Poultry Science, University of Arkansas, Fayetteville 72701, USA.
Poult Sci. 1996 Mar;75(3):351-6. doi: 10.3382/ps.0750351.
Smyth line (SL) chickens spontaneously develop a posthatch autoimmune loss of pigment cells (vitiligo) in the feather. Concurrent with the development of Smyth line vitiligo (SLV), mononuclear cell infiltration and altered T cell profiles can be observed in the pulp of developing feathers. To determine whether the development of SLV is preceded by or associated with alterations in blood lymphocyte and leukocyte populations, blood leukocyte profiles were established at various times prior to and throughout the spontaneous development of SLV. The proportions among various blood leukocyte populations (percentage of lymphocytes, monocytes, heterophils, eosinophils, and basophils) were determined by immunofluorescence and flow cytometric analyses. Lymphocyte markers included fluorescence-conjugated monoclonal antibodies to identify T cells (CD3), T helper cells (CD4), cytotoxic T cells (CD8), and B cells (IgM). The proportions among blood lymphocyte populations examined did not differ between SL and MCH-matched parental Brown line (BL) control chickens prior to and throughout the development of SLV. Compared to BL controls, SL chickens had, however, increased proportions of inflammatory leukocytes in the blood, particularly at the time when most hatchmates developed SLV. Examination of leukocyte alterations with respect to first observation of SLV revealed that inflammatory leukocyte levels were elevated early in SLV. Although altered leukocyte profiles in the blood were observed during the development of SLV, blood from SL chickens did not reflect alterations in lymphocyte populations known to occur at the site of melanocyte destruction. The role of inflammatory blood leukocytes in the development of SLV needs to be further investigated.
斯迈思系（SL）鸡在孵化后会自发出现羽毛色素细胞的自身免疫性丧失（白癜风）。在斯迈思系白癜风（SLV）发展的同时，在发育中的羽毛髓质中可观察到单核细胞浸润和T细胞谱改变。为了确定SLV的发展是否先于或与血液淋巴细胞和白细胞群体的改变相关，在SLV自发发展之前及整个过程中的不同时间点建立了血液白细胞谱。通过免疫荧光和流式细胞术分析确定了各种血液白细胞群体之间的比例（淋巴细胞、单核细胞、异嗜性粒细胞、嗜酸性粒细胞和嗜碱性粒细胞的百分比）。淋巴细胞标志物包括荧光偶联单克隆抗体，用于识别T细胞（CD3）、辅助性T细胞（CD4）、细胞毒性T细胞（CD8）和B细胞（IgM）。在SLV发展之前及整个过程中，所检测的血液淋巴细胞群体比例在SL系和与MCH匹配的亲本布朗系（BL）对照鸡之间没有差异。然而，与BL对照相比，SL鸡血液中炎症白细胞的比例增加，特别是在大多数同窝鸡出现SLV的时候。关于首次观察到SLV时白细胞变化的检查显示，SLV早期炎症白细胞水平升高。虽然在SLV发展过程中观察到血液中白细胞谱发生了改变，但SL鸡的血液并未反映出已知在黑素细胞破坏部位发生的淋巴细胞群体变化。炎症血液白细胞在SLV发展中的作用需要进一步研究。
