Spector A, Wang G M, Wang R R, Li W C, Kuszak J R
Department of Ophthalmology, College of Physicians & Surgeons of Columbia University, New York, NY 10032, USA.
Exp Eye Res. 1995 May;60(5):471-81. doi: 10.1016/s0014-4835(05)80062-4.
Short-term photochemical insult of cultured rat lenses caused by the generation of H2O2, O2<--and OH. was found to lead to rapid irreversible damage to the epithelial cell layer. This irreversible damage was measured by Trypan blue staining, terminal deoxyribonucleotidyl transferase labeling, DNA laddering and morphological analyses. There appears to be an inverse relationship between the period of photochemical insult and the post-insult time required to observe epithelial cell damage. Insulting periods of a few hours require post-insult intervals of days to observe significant cell damage and weeks before complete cortical cataracts are found. Epithelial cell damage precedes the loss of transparency.
由过氧化氢、超氧阴离子和羟基的产生所导致的培养大鼠晶状体的短期光化学损伤，被发现会导致上皮细胞层迅速发生不可逆损伤。这种不可逆损伤通过台盼蓝染色、末端脱氧核苷酸转移酶标记、DNA梯状条带分析和形态学分析来测定。光化学损伤的时长与观察到上皮细胞损伤所需的损伤后时间之间似乎存在反比关系。数小时的损伤时长需要损伤后数天的间隔才能观察到明显的细胞损伤，而在发现完全的皮质性白内障之前则需要数周时间。上皮细胞损伤先于透明度丧失。
