Varma S D, Mooney J M
J Free Radic Biol Med. 1986;2(1):57-62. doi: 10.1016/0748-5514(86)90124-8.
Studies have been conducted to examine the implications of photochemical generation of O2- and its derivatization to H2O2 and OH . in the physiology of the lens in vitro. Physiological status was determined by measuring the uptake of rubidium by the intact tissue when cultured in riboflavin-containing medium, in dark and light, and in the presence and absence of various scavengers. In the presence of light, the uptake of rubidium in the lens was greatly diminished; this suggests photodamage to the tissue. MnSOD and ferricyanide protected against this photochemical damage. The damaging process was thus initiated by the generation of O2-. The tissue damage was also attenuated by catalase, ferrocyanide, and mannitol. These results, therefore, suggest the participation of hydrogen peroxide and the subsequent Haber-Weiss reaction in the photodamaging process.
已经开展了多项研究，以探讨体外晶状体生理学中光化学产生超氧阴离子（O2-）及其衍生为过氧化氢（H2O2）和羟基自由基（OH·）的影响。通过测量完整组织在含有核黄素的培养基中培养时，在黑暗和光照条件下以及存在和不存在各种清除剂的情况下铷的摄取量来确定生理状态。在光照条件下，晶状体中铷的摄取量大大减少；这表明组织受到了光损伤。超氧化物歧化酶（MnSOD）和铁氰化物可防止这种光化学损伤。因此，损伤过程是由超氧阴离子的产生引发的。过氧化氢酶、亚铁氰化物和甘露醇也减轻了组织损伤。因此，这些结果表明过氧化氢以及随后的哈伯-维伊斯（Haber-Weiss）反应参与了光损伤过程。
