Sortwell C E, Pappas G D, Sagen J
Department of Anatomy and Cell Biology, University of Illinois at Chicago 60612, USA.
Exp Brain Res. 1995;103(1):59-69. doi: 10.1007/BF00241965.
Adrenal medullary allografts, as well as other monoaminergic tissues, have been demonstrated in our laboratory to increase antidepressive activity when transplanted into the frontal neocortex of rats. Refinement in the optimal parameters for xenograft viability has indicated that isolated bovine chromaffin cells may be an improved source of graft donor tissue. The aim of the present study was to determine whether isolated bovine chromaffin cell grafts to the rat frontal neocortex could provide an alternative source of catecholamines for antidepressant activity. Isolated bovine chromaffin cells, isolated bovine fibroblasts, or an equal volume of vehicle were unilaterally implanted into the right or left frontal cortex or right visual cortex. All rats were assessed before and 6 weeks after transplantation using the forced swimming test, a popular measure of antidepressant activity. Bovine chromaffin cell grafts in either the right or left frontal cortex produced significant increases in antidepressant activity compared to grafts of bovine fibroblasts and sham-operated or nontransplanted rats. In contrast, bovine chromaffin cells transplanted to the visual cortex did not affect antidepressant activity. Bovine fibroblast grafts in the frontal cortex also induced slight increases in antidepressant activity, although significantly less than chromaffin cell grafts. Morphological analysis revealed robust survival of tyrosine hydroxylase-positive chromaffin cells that retained their in situ ultrastructure and occasionally formed synaptic connections with the host parenchyma. These results suggest that xenografted isolated bovine chromaffin cells can provide a viable source of catecholamines for antidepressive activity.
在我们实验室已证实，肾上腺髓质同种异体移植以及其他单胺能组织移植到大鼠额叶新皮质后，会增强抗抑郁活性。对异种移植存活最佳参数的优化表明，分离的牛嗜铬细胞可能是更好的移植供体组织来源。本研究的目的是确定将分离的牛嗜铬细胞移植到大鼠额叶新皮质是否能为抗抑郁活性提供一种替代的儿茶酚胺来源。将分离的牛嗜铬细胞、分离的牛成纤维细胞或等体积的赋形剂单侧植入右侧或左侧额叶皮质或右侧视皮质。所有大鼠在移植前及移植后6周使用强迫游泳试验进行评估，这是一种常用的抗抑郁活性检测方法。与牛成纤维细胞移植组以及假手术或未移植的大鼠相比，右侧或左侧额叶皮质植入牛嗜铬细胞均能显著增强抗抑郁活性。相比之下，移植到视皮质的牛嗜铬细胞对抗抑郁活性没有影响。额叶皮质植入牛成纤维细胞也会使抗抑郁活性略有增加，尽管明显低于嗜铬细胞移植组。形态学分析显示酪氨酸羟化酶阳性的嗜铬细胞存活良好，保留了其原位超微结构，偶尔还与宿主实质形成突触连接。这些结果表明，异种移植的分离牛嗜铬细胞可为抗抑郁活性提供一种可行的儿茶酚胺来源。
