Sugahara T, Shirahata S, Sasaki T, Murakami H
Department of Biotechnology, College of Agriculture, Ehime University, Japan.
FEBS Lett. 1995 Jul 10;368(1):92-6. doi: 10.1016/0014-5793(95)00607-b.
Immunoglobulin production stimulating factor-II alpha, which enhances immunoglobulin production of human and mouse hybridomas was purified from cell lysate of human Burkitt's lymphoma, Namalwa cells, and identified as glyceraldehyde-3-phosphate dehydrogenase. The enhancement of immunoglobulin production with this enzyme was not linked with its enzymatic activity. The enzyme enhanced immunoglobulin productivity of transcription-suppressed hybridomas, but did not enhance that of translation-suppressed hybridomas. From these results, it is suggested that this enzyme takes part in the post-translational control or the enhancement of translation activity to stimulate immunoglobulin production of hybridomas.
免疫球蛋白产生刺激因子-IIα，可增强人和小鼠杂交瘤的免疫球蛋白产生，从人伯基特淋巴瘤Namalwa细胞的细胞裂解物中纯化得到，并鉴定为甘油醛-3-磷酸脱氢酶。该酶对免疫球蛋白产生的增强作用与其酶活性无关。该酶增强了转录抑制杂交瘤的免疫球蛋白生产力，但未增强翻译抑制杂交瘤的免疫球蛋白生产力。从这些结果推测，该酶参与翻译后调控或增强翻译活性以刺激杂交瘤的免疫球蛋白产生。
