Tanahashi M, Yao T, Kokubo T, Minoda M, Miyamoto T, Nakamura T, Yamamuro T
Division of Material Chemistry, Faculty of Engineering, Kyoto University, Japan.
J Biomed Mater Res. 1995 Mar;29(3):349-57. doi: 10.1002/jbm.820290310.
A dense, uniform, and highly biologically active bone-like apatite layer can be formed in arbitrary thickness on any kind and shape of solid substrate surface by the following biomimetic method at ordinary temperature and pressure: First, a substrate is set in contact with particles of bioactive CaO-SiO2-based glass soaked in a simulated body fluid (SBF) with inorganic ion concentrations nearly equal to those of human blood plasma so that a number of apatite nuclei are formed on the substrate. Second, the substrate is soaked in another solution with ion concentrations 1.5 times those of SBF (1.5SBF) so that the apatite nuclei grow in situ. In the present study, organic polymer substrates were treated with glow-discharge in O2 gas atmosphere, then subjected to the above-mentioned biomimetic process. The induction periods for the apatite nucleation on all the examined organic polymers were reduced from 24 to 6 h, with glow-discharge treatment. The adhesive strengths of the formed apatite layer to the substrates increased from about 4 to 10 MPa for poly(ethylene terephthalate) and poly-ether sulfone, and from 1 approximately 2 to 6 approximately 7 MPa even for poly(methyl methacrylate), polyamide 6 and polyethylene. It is supposed that highly polar groups such as carbonyl, ester, hydroxyl, and carboxyl ones formed by glow-discharge treatment increased the affinity of a silicate ion with the substrates to decrease the induction period, and also increased the affinity of the apatite with the substrate to increase the adhesive strength.(ABSTRACT TRUNCATED AT 250 WORDS)
在常温常压下，通过以下仿生方法可在任何种类和形状的固体基质表面形成任意厚度的致密、均匀且具有高生物活性的骨样磷灰石层：首先，将基质与浸泡在模拟体液（SBF）中的生物活性CaO-SiO₂基玻璃颗粒接触，该模拟体液的无机离子浓度几乎与人体血浆相同，从而在基质上形成许多磷灰石晶核。其次，将基质浸泡在另一种离子浓度为SBF的1.5倍（1.5SBF）的溶液中，使磷灰石晶核原位生长。在本研究中，有机聚合物基质在O₂气体气氛中进行辉光放电处理，然后进行上述仿生过程。经过辉光放电处理后，所有检测的有机聚合物上磷灰石成核的诱导期从24小时缩短至6小时。对于聚对苯二甲酸乙二酯和聚醚砜，形成的磷灰石层与基质的粘附强度从约4MPa增加到10MPa，对于聚甲基丙烯酸甲酯、聚酰胺6和聚乙烯，甚至从约1至2MPa增加到6至7MPa。据推测，辉光放电处理形成的羰基、酯基、羟基和羧基等高极性基团增加了硅酸盐离子与基质的亲和力，从而缩短了诱导期，同时也增加了磷灰石与基质的亲和力，提高了粘附强度。（摘要截短于250字）
