Tanahashi M, Yao T, Kokubo T, Minoda M, Miyamoto T, Nakamura T, Yamamuro T
Division of Material Chemistry, Faculty of Engineering, Kyoto University, Japan.
J Appl Biomater. 1994 Winter;5(4):339-47. doi: 10.1002/jab.770050409.
A dense, uniform and highly biologically active bone-like apatite layer can be formed in arbitrary thickness on any kind and shape of solid substrate surface by the following biomimetic method at ordinary temperature and pressure. First, a substrate is set in contact with particles of bioactive CaO SiO2 based glass soaked in a simulated body fluid (SBF) with inorganic ion concentrations nearly equal to those of human blood plasma. Second, the substrate is soaked in another solution with ion concentrations 1.5 times those of SBF (1.5 SBF). In the present study, organic polymer substrates treated with 5 M NaOH solution were subjected to the above mentioned biomimetic process. The induction periods for the apatite nucleation on polyethyleneterephthalate (PET), polymethylmethacrylate (PMMA), polyamide 6 (PA6), and polyethersulfone (PESF) substrates were reduced from 24 to 12 h with the NaOH treatment. The adhesive strength of the formed apatite layer were increased from 3.5 to 8.6 MPa, from 1.1 to 3.4 MPa, and from 0.6 to 5.3 MPa with the NaOH treatment, for PET, PMMA, and PA 6, respectively. It was assumed that highly polar groups, such as carboxyl and sulfinyl ones formed by the hydrolysis of an ester group on PET and PMMA and of an amide group on PA 6, or of a sulfonyl group on PESF with the NaOH treatment, attached a large number of hydrated silica dissolved from the glass particles, to accelerate the apatite nucleation, and also to form a strong bond with the apatite. The apatite-organic polymer composites thus obtained are expected to be useful as bone-repairing as well as soft tissue-repairing materials.
在常温常压下，通过以下仿生方法可在任何种类和形状的固体基材表面形成任意厚度的致密、均匀且具有高生物活性的骨样磷灰石层。首先，将基材与浸泡在无机离子浓度几乎与人体血浆相同的模拟体液（SBF）中的生物活性CaO SiO₂基玻璃颗粒接触。其次，将基材浸泡在离子浓度为SBF 1.5倍的另一种溶液（1.5 SBF）中。在本研究中，用5 M NaOH溶液处理过的有机聚合物基材进行上述仿生过程。经NaOH处理后，聚对苯二甲酸乙二酯（PET）、聚甲基丙烯酸甲酯（PMMA）、聚酰胺6（PA6）和聚醚砜（PESF）基材上磷灰石成核的诱导期从24小时缩短至12小时。对于PET、PMMA和PA6，经NaOH处理后，形成的磷灰石层的粘结强度分别从3.5 MPa提高到8.6 MPa、从1.1 MPa提高到3.4 MPa以及从0.6 MPa提高到5.3 MPa。据推测，通过NaOH处理，PET和PMMA上的酯基、PA6上的酰胺基或PESF上的磺酰基水解形成的羧基和亚磺酰基等高极性基团，附着了大量从玻璃颗粒溶解的水合二氧化硅，从而加速磷灰石成核，并与磷灰石形成牢固的键。由此获得的磷灰石 - 有机聚合物复合材料有望用作骨修复以及软组织修复材料。
