Vythilingam I, Oda K, Chew T K, Mahadevan S, Vijayamalar B, Morita K, Tsuchie H, Igarashi A
Institute for Medical Research, Kuala Lumpur, Malaysia.
J Am Mosq Control Assoc. 1995 Mar;11(1):94-8.
Detection and isolation of Japanese encephalitis (JE) virus were attempted from female mosquitoes collected in Kampong Pasir Panjang, Sabak Bernam, Selangor, from May to November 1992. A total of 7,400 mosquitoes consisting of 12 species in 148 pools were processed and inoculated into Aedes albopictus clone C6/36 cell cultures. Of these, 26 pools showed the presence of viral antigens in the infected C6/36 cells by specific immunoperoxidase staining using an anti-JE virus polyclonal antibody. Presence of JE virus genome was confirmed in the infected culture fluid for 16 pools by using reverse transcriptase-polymerase chain reaction and JE virus-specific primers. Of these, 3 pools were from Culex tritaeniorhynchus, 4 from Culex vishnui, 3 from Culex bitaeniorhynchus, 2 from Culex sitiens, one from Aedes species, and 3 from Culex species. Isolation of JE virus from Cx. sitiens, Cx. bitaeniorhynchus, and Aedes sp. (Aedes butleri and Ae. albopictus) is reported for the first time in Malaysia.
1992年5月至11月期间，研究人员尝试从雪兰莪州沙白安南甘榜巴西淡港收集的雌蚊中检测和分离日本脑炎（JE）病毒。总共对148个样本池中的7400只蚊子（共12个种类）进行了处理，并接种到白纹伊蚊克隆C6/36细胞培养物中。其中，通过使用抗JE病毒多克隆抗体的特异性免疫过氧化物酶染色，有26个样本池在感染的C6/36细胞中显示出病毒抗原的存在。通过使用逆转录聚合酶链反应和JE病毒特异性引物，在16个样本池的感染培养液中证实了JE病毒基因组的存在。其中，3个样本池来自三带喙库蚊，4个来自致倦库蚊，3个来自二带喙库蚊，2个来自海滨库蚊，1个来自伊蚊属，3个来自库蚊属。在马来西亚首次报道了从海滨库蚊、二带喙库蚊和伊蚊属（布氏伊蚊和白纹伊蚊）中分离出JE病毒。
