Vythilingam I, Oda K, Tsuchie H, Mahadevan S, Vijayamalar B
Institute for Medical Research, Jalan Pahang, Kuala Lumpur, Malaysia.
J Am Mosq Control Assoc. 1994 Jun;10(2 Pt 1):228-9.
Isolation of Japanese encephalitis virus (JEV) from mosquitoes in Sabak Bernam, Selangor, Malaysia, was attempted. An aliquot of homogenate from each pool of mosquitoes, 50 per tube, was inoculated into Aedes albopictus clone C6/36 cells for virus isolation. Each cell culture was tested for the presence of viral antigen by immunoperoxidase staining using an anti-JEV polyclonal antibody. Out of 4 Culex sitiens mosquito pools, 2 pools were positive for JEV by cell culture. Presence of JEV genome in the cell cultures for Cx. sitiens was confirmed by using reverse transcriptase-polymerase chain reaction and JEV-specific primers. This is the first report on the isolation of JEV from Cx. sitiens.
尝试从马来西亚雪兰莪州沙白安南的蚊子中分离日本脑炎病毒（JEV）。将每管50只蚊子的匀浆等分试样接种到白纹伊蚊克隆C6/36细胞中进行病毒分离。使用抗JEV多克隆抗体通过免疫过氧化物酶染色检测每个细胞培养物中病毒抗原的存在。在4个致倦库蚊蚊子样本中，有2个样本通过细胞培养检测出JEV呈阳性。使用逆转录聚合酶链反应和JEV特异性引物证实了致倦库蚊细胞培养物中存在JEV基因组。这是关于从致倦库蚊中分离出JEV的首次报告。
