Terlikowski S, Nowak H F, Lotocki W
Department of Gynecology and Septic Obstetrics, Medical School, Białystok, Poland.
Neoplasma. 1995;42(2):83-7.
The effect of human recombinant tumor necrosis factor alpha (h rec TNF-alpha) on the growth of Morris hepatoma 5123 implanted in the skeletal muscles of the thigh of Buffalo rats was investigated. The cytokine was repeatedly given in an intratumor administration (i.t.) in dose of 1.5 x 10(4) U once a day in regimens of four or eight days. Comparative groups consisted of animals which were given saline i.t. Control groups included healthy rats subjected to local cytokine effect. The experiments revealed an inhibitory effects of the preparation on the growth of tumors. Biometric parameters of the tumors induced indicated that the inhibition of Morris hepatoma was most effective after the eighth dose of h rec TNF-alpha. The administration of fourfold dose resulted in an initial loss of body mass increase. However, when injected eight times, the factor produced a relative tolerance reflected in minor reduction of actual body mass. The estimation of survival time in rats injected i.t. with h rec TNF-alpha, compared to those given saline, revealed statistically significant differences at the eighth repeated dose.
研究了重组人肿瘤坏死因子α（h rec TNF-α）对植入水牛大鼠大腿骨骼肌的Morris肝癌5123生长的影响。该细胞因子通过瘤内给药（i.t.），以1.5×10⁴ U的剂量每天一次，给药方案为4天或8天。比较组包括接受瘤内注射生理盐水的动物。对照组包括接受局部细胞因子作用的健康大鼠。实验揭示了该制剂对肿瘤生长的抑制作用。诱导肿瘤的生物统计学参数表明，在第八次注射h rec TNF-α后，对Morris肝癌的抑制最为有效。四倍剂量的给药导致体重增加最初减少。然而，当注射八次时，该因子产生了相对耐受性，表现为实际体重略有下降。与注射生理盐水的大鼠相比，对瘤内注射h rec TNF-α的大鼠的存活时间进行估计，发现在第八次重复给药时有统计学上的显著差异。
