Terlikowski S, Nowak H F, Sulkowska M, Sulkowski S
Department of Gynecology and Septic Obstetrics, Medical School, Bialystok, Poland.
Neoplasma. 1996;43(5):327-33.
The experiment used Morris hepatoma 5123 series growing in muscles of the right hind limb of Buffalo rats. The group I animals were given intratumor 4 doses of TNF-alpha and group II-8 doses of TNF-alpha (10 micrograms/day). Control groups (III and IV) consisted of rats with injected Morris hepatoma, which were given PBS solution instead of TNF-alpha. A decrease in the volume of neoplastic metastases was observed in groups I and II, compared with groups III and IV. At the same time an increase was found in the volume of metastatic tumors in group II (8 x TNF-alpha), compared with group I (4 x TNF-alpha). Histological and ultrastructural analysis of the pulmonary tissue revealed intensified fibrotic reactions and inflammatory infiltrations around the metastatic tumors. The change were much more enhanced in group II, which might affect the results of neoplastic metastatic volume measurements. We concluded that multiple human recombinant TNF-alpha, hrec TNF-alpha, local injections inhibited dissemination of tumor cells and prolonged the survival time of rats up to the 76th day of the follow-up.
该实验使用了生长在水牛大鼠右后肢肌肉中的莫里斯肝癌5123系列。第一组动物接受4剂肿瘤内注射的肿瘤坏死因子-α（TNF-α），第二组接受8剂TNF-α（10微克/天）。对照组（第三组和第四组）由注射了莫里斯肝癌的大鼠组成，它们接受的是磷酸盐缓冲盐水（PBS）溶液而非TNF-α。与第三组和第四组相比，第一组和第二组中肿瘤转移灶的体积减小。同时，与第一组（4剂TNF-α）相比，第二组（8剂TNF-α）中转移瘤的体积增大。肺组织的组织学和超微结构分析显示，转移瘤周围的纤维化反应和炎症浸润增强。第二组中的这些变化更为明显，这可能会影响肿瘤转移体积测量的结果。我们得出结论，多次局部注射重组人肿瘤坏死因子-α（hrec TNF-α）可抑制肿瘤细胞的扩散，并将大鼠的生存时间延长至随访的第76天。
