Sulkowska M, Sulkowski S, Terlikowski S, Nowak H F
Department of Pathological Anatomy, Medical Academy of Białystok.
Rocz Akad Med Bialymst. 1997;42 Suppl 1:354-62.
The aim of this study was to explore the effect of intratumour mutein VI-Hrec TNF-alpha administration upon the ultrastructural changes within the pulmonary tissue, with special attention paid to type II alveolar epithelial cells. The experiment was carried out on Buffalo rats with implantable Morris hepatoma series 5123 in the skeletal muscles of the limb. Mutein VI-Hrec TNF-alpha was administered in a dose of 10 micrograms once a day in a cycle of eight days. Control animals were given saline (PBS). Ultrastructural changes within the pulmonary tissue were evaluated in the electron transmission microscope (TEM), with special attention paid to alveolar epithelial cells. In the animals receiving hrec TNF-alpha mutein VI, damage to the alveolar epithelial cells was found. In the later period (14 days after the mutein treatment), repairing processes were observed, accompanied by intensified fibrotic processes in the interalveolar septal interstitium, with the subsequent pulmonary tissue rebuilding. The study confirmed the possibility of a peripheral action of hrec TNF-alpha mutein VI after its administration to the experimental Morris hepatoma and found the alveolar epithelial cells to be a key element of the pulmonary tissue subjected to the cytokine effect.
本研究的目的是探讨瘤内给予突变体VI-Hrec TNF-α对肺组织超微结构变化的影响，特别关注II型肺泡上皮细胞。实验在布法罗大鼠身上进行，这些大鼠的肢体骨骼肌植入了可移植的莫里斯肝癌5123。以每天10微克的剂量给予突变体VI-Hrec TNF-α，为期8天。对照组动物给予生理盐水（PBS）。在电子透射显微镜（TEM）下评估肺组织的超微结构变化，特别关注肺泡上皮细胞。在接受hrec TNF-α突变体VI的动物中，发现肺泡上皮细胞受损。在后期（突变体治疗后14天），观察到修复过程，同时肺泡间隔间质中的纤维化过程加剧，随后肺组织进行重建。该研究证实了将hrec TNF-α突变体VI给予实验性莫里斯肝癌后其外周作用的可能性，并发现肺泡上皮细胞是受细胞因子作用的肺组织的关键要素。
