Alonso A, Meirelles N C, Tabak M
Instituto de Fisica, Universidade Estadual de Campinas, Säo Paulo, Brazil.
Physiol Chem Phys Med NMR. 1995;27(1):63-72.
Intercellular membranes of Stratum Corneum and erythrocyte ghosts were studied through partition of Tempo nitroxide spin label. Stratum Corneum presents a phase transition-monitored by the spin label at 58 degrees C--that is in agreement with data obtained through the use of other techniques. A reduction of the nitroxide is observed. Erythrocyte ghosts at protein concentrations higher than the values generally used in ESR studies (above 15 mg/ml) also show the partition and reduction of Tempo. Experimental data suggest that the reduction mechanism might be common to both types of membranes involving probably the membrane lipoperoxidation as well as protein oxidation.
通过Tempo氮氧化物自旋标记的分配对角质层和红细胞血影的细胞间膜进行了研究。角质层呈现出一个相变——由自旋标记在58摄氏度监测——这与通过使用其他技术获得的数据一致。观察到氮氧化物的减少。蛋白质浓度高于电子顺磁共振研究中通常使用的值（高于15毫克/毫升）的红细胞血影也显示出Tempo的分配和减少。实验数据表明，还原机制可能在这两种类型的膜中是共同的，可能涉及膜脂质过氧化以及蛋白质氧化。
