不同人群中DNA加合物的32P后标记分析。

32P-postlabeling analysis of DNA adducts in different populations.

作者信息

Schell C, Popp W, Kraus R, Vahrenholz C, Norpoth K

机构信息

Institute of Hygiene and Occupational Medicine, University Medical Center, Essen, Federal Republic of Germany.

出版信息

Toxicol Lett. 1995 May;77(1-3):299-307. doi: 10.1016/0378-4274(95)03310-6.

DOI:10.1016/0378-4274(95)03310-6

PMID:7618154

Abstract

Blood samples were obtained from different populations exposed occupationally or by lifestyle habits to polycyclic aromatic hydrocarbons (PAH). DNA adducts were determined by 32P-postlabeling assay either in white blood cells (WBC) or lymphocytes. The level of DNA adducts ranged from 1.5 per 10(9) nucleotides in one of the control groups up to 7.1 per 10(9) nucleotides in one group of PAH-exposed workers. Comparison of the adduct thin layer chromatography (TLC) profiles revealed individual variation in both pattern and level of DNA adducts. Significant differences of adduct levels were detected between one group of PAH-exposed coke-oven workers and the corresponding control group. Only a weak influence of the smoking habits on the amount of adducts was detectable in occupationally exposed or unexposed individuals.

摘要

从因职业或生活方式习惯接触多环芳烃（PAH）的不同人群中采集血样。通过32P后标记分析法测定白细胞（WBC）或淋巴细胞中的DNA加合物。DNA加合物水平在一个对照组中为每10⁹个核苷酸1.5个，在一组接触PAH的工人中高达每10⁹个核苷酸7.1个。加合物薄层色谱（TLC）图谱的比较显示，DNA加合物的模式和水平存在个体差异。在一组接触PAH的焦炉工人与相应对照组之间检测到加合物水平的显著差异。在职业暴露或未暴露个体中，仅可检测到吸烟习惯对加合物量的微弱影响。