Poli A, Abramo F, Matteucci D, Baldinotti F, Pistello M, Lombardi S, Barsotti P, Bendinelli M
Department of Animal Pathology, University of Pisa, Italy.
Vet Immunol Immunopathol. 1995 May;46(1-2):13-20. doi: 10.1016/0165-2427(94)07002-o.
Renal alterations characterized morphologically by glomerular and tubulo-interstitial lesions and clinically by a heavy proteinuria and sometimes by renal failure are frequent in feline immunodeficiency virus (FIV) infected cats. To investigate the possible role of local FIV replication in the genesis of this renal damage, renal tissues of 15 consecutive naturally infected and five non-infected cats were examined for traces of the virus by immunohistochemistry, using a monoclonal anti-p24 antibody in a streptavidin-biotin peroxidase labeled system, cultivation and polymerase chain reaction (PCR). Tubular epithelial cells as well as scattered interstitial inflammatory and glomerular cells were positive for p24 antigen in 13 cats. Viral isolation was successful in seven cats, and FIV gag DNA and RNA sequences were detected in 14 and five cats, respectively. Control cats were constantly negative. Although not conclusive, these results suggest that a direct role of FIV in the induction of the renal damage observed in infected animals is possible.
猫免疫缺陷病毒（FIV）感染的猫经常出现肾脏改变，其形态学特征为肾小球和肾小管间质病变，临床特征为重度蛋白尿，有时还会出现肾衰竭。为了研究局部FIV复制在这种肾脏损伤发生过程中可能发挥的作用，我们采用链霉亲和素-生物素过氧化物酶标记系统中的单克隆抗p24抗体，通过免疫组织化学、培养和聚合酶链反应（PCR），对15只连续自然感染的猫和5只未感染的猫的肾脏组织进行了病毒痕迹检测。13只猫的肾小管上皮细胞以及散在的间质炎性细胞和肾小球细胞的p24抗原呈阳性。7只猫病毒分离成功，14只和5只猫分别检测到FIV gag DNA和RNA序列。对照猫始终为阴性。尽管这些结果尚无定论，但提示FIV在感染动物中观察到的肾脏损伤诱导过程中可能直接起作用。
