del Fierro G M, Bundesen P, Martin S, Jones S, Beetson S, Robinson W F
School of Veterinary Studies, Murdoch University, Australia.
Vet Immunol Immunopathol. 1995 May;46(1-2):93-101. doi: 10.1016/0165-2427(94)07009-v.
A commercially available whole blood agglutination test, VetRED FIV, used for the detection of antibodies to feline immunodeficiency virus (FIV), was evaluated. The test is based on the use of a synthetic peptide conjugated to a non-agglutinating anti-feline red blood cell monoclonal antibody. The amino acid sequence of the synthetic peptide was derived from the predicted sequence of the transmembrane protein of FIV. The sensitivity and specificity of VetRED FIV was 100% when 34 known FIV-positive and 15 known FIV-negative cats were tested. These cats were part of studies on experimentally induced FIV infection, with their FIV status confirmed by virus isolation. Further, VetRED FIV was compared with another commercially available test for FIV antibody, PetChek in a field trial on 548 feline blood samples received by a diagnostic laboratory. Of the test results 94.2% (516/548) were in agreement: 112 were positive by VetRED FIV and PetChek; 404 were negative by both tests and 32 were discordant. These 5.8% discordant samples producing VetRED FIV-positive/PetChek-negative or VetRED FIV-negative/PetChek-positive were further assessed by Western blot assay. In the field trial, the sensitivity and specificity of VetRED FIV was 97% and 97%, respectively, comparable to the 98% sensitivity and 99% specificity for PetChek. The results from the trial also confirm the relatively high overall prevalence of FIV in Australian cats predominantly among mature male cats in the 9-12 year age group. Given the simplicity of the VetRED FIV procedure, it is concluded that VetRED FIV is a useful addition to the available commercial tests for FIV infection.
对一种用于检测猫免疫缺陷病毒（FIV）抗体的市售全血凝集试验VetRED FIV进行了评估。该试验基于使用与非凝集性抗猫红细胞单克隆抗体偶联的合成肽。合成肽的氨基酸序列源自FIV跨膜蛋白的预测序列。对34只已知FIV阳性和15只已知FIV阴性的猫进行检测时，VetRED FIV的敏感性和特异性均为100%。这些猫是实验性诱导FIV感染研究的一部分，其FIV状态通过病毒分离得到确认。此外，在一项针对诊断实验室收到的548份猫血样的现场试验中，将VetRED FIV与另一种市售的FIV抗体检测方法PetChek进行了比较。检测结果中94.2%（516/548）一致：112份经VetRED FIV和PetChek检测均为阳性；404份两种检测均为阴性，32份结果不一致。对这5.8%产生VetRED FIV阳性/PetChek阴性或VetRED FIV阴性/PetChek阳性的不一致样本，通过蛋白质印迹分析进一步评估。在现场试验中，VetRED FIV的敏感性和特异性分别为97%和97%，与PetChek的98%敏感性和99%特异性相当。试验结果还证实，FIV在澳大利亚猫中的总体患病率相对较高，主要集中在9至12岁年龄组的成年雄性猫中。鉴于VetRED FIV操作简单，得出结论：VetRED FIV是现有FIV感染商业检测方法的有益补充。
