Wilson K M, Robinson W F, Mitchelhill K I, Bundesen P G, Kemp B E
St. Vincent's Institute of Medical Research, Fitzroy, Vic., Australia.
Vet Immunol Immunopathol. 1994 Sep;42(3-4):253-63. doi: 10.1016/0165-2427(94)90071-x.
The T-lymphotropic lentivirus, feline immunodeficiency virus (FIV) is now recognised as a major viral pathogen affecting domestic cat populations worldwide. A rapid, autologous red cell agglutination test for antibodies to FIV has been developed. A synthetic peptide analog corresponding to the immunodominant epitope within the FIV transmembrane glycoprotein gp40 residues (680-715) KVEAMEKFLYTAFAMQELGC (Acm)NQNQFFK(BrAc)KIPLELWTR was conjugated to an anti-feline erythrocyte antibody using a thio-ether linkage. Within 3 min of adding this reagent to 20 microliters of whole blood, circulating antibody to the peptide epitope caused agglutination of the red blood cells. The performance of this simple test is comparable with the two commercially available enzyme immunoassay (EIA) kits and an EIA based on this peptide. A variant of the gp40 (680-715) peptide corresponding to the FIV, PPR strain gp40 (678-716) sequence was also synthesised and no difference in reactivity was observed in an EIA on 211 seropositive samples, indicating that the peptide-based test may be applicable to other known strains of the virus.
亲嗜T淋巴细胞的慢病毒——猫免疫缺陷病毒（FIV），如今被公认为是影响全球家猫群体的一种主要病毒病原体。现已开发出一种针对FIV抗体的快速、自体红细胞凝集试验。一种与FIV跨膜糖蛋白gp40残基（680 - 715）内的免疫显性表位相对应的合成肽类似物KVEAMEKFLYTAFAMQELGC（Acm）NQNQFFK（BrAc）KIPLELWTR，通过硫醚键与抗猫红细胞抗体偶联。将该试剂加入20微升全血后3分钟内，针对该肽表位的循环抗体导致红细胞凝集。这个简单试验的性能与两种市售酶免疫测定（EIA）试剂盒以及基于该肽的一种EIA相当。还合成了与FIV的PPR株gp40（678 - 716）序列相对应的gp40（680 - 715）肽变体，在对211份血清阳性样本进行的EIA中未观察到反应性差异，这表明基于肽的试验可能适用于该病毒的其他已知毒株。
