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压力稳定性并非嗜热酶的普遍特性:沃氏甲烷球菌、泥生甲烷球菌、嗜热栖热甲烷球菌和詹氏甲烷球菌的腺苷酸激酶。

Pressure stabilization is not a general property of thermophilic enzymes: the adenylate kinases of Methanococcus voltae, Methanococcus maripaludis, Methanococcus thermolithotrophicus, and Methanococcus jannaschii.

作者信息

Konisky J, Michels P C, Clark D S

机构信息

Department of Microbiology, University of Illinois, Urbana 61801, USA.

出版信息

Appl Environ Microbiol. 1995 Jul;61(7):2762-4. doi: 10.1128/aem.61.7.2762-2764.1995.

Abstract

The application of 50-MPa pressure did not increase the thermostabilities of adenylate kinases purified from four related mesophilic and thermophilic marine methanogens. Thus, while it has been reported that some thermophilic enzymes are stabilized by pressure (D. J. Hei and D. S. Clark, Appl. Environ. Microbiol. 60:932-939, 1994), hyperbaric stabilization is not an intrinsic property of all enzymes from deep-sea thermophiles.

摘要

50兆帕压力的施加并未提高从四种相关嗜温及嗜热海洋产甲烷菌中纯化得到的腺苷酸激酶的热稳定性。因此,尽管已有报道称一些嗜热酶可通过压力实现稳定(D. J. 黑伊和D. S. 克拉克,《应用与环境微生物学》60:932 - 939,1994年),但高压稳定并非所有深海嗜热菌酶的固有特性。

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