脉冲场凝胶电泳技术在食源性大肠杆菌O157:H7暴发流行期间的流行病学研究中的应用
Use of pulsed-field gel electrophoresis for epidemiological study of Escherichia coli O157:H7 during a food-borne outbreak.
作者信息
Johnson J M, Weagant S D, Jinneman K C, Bryant J L
机构信息
Seattle District Laboratory, Food and Drug Administration, Bothell, Washington 98041, USA.
出版信息
Appl Environ Microbiol. 1995 Jul;61(7):2806-8. doi: 10.1128/aem.61.7.2806-2808.1995.
Abstract
Food and patient isolates from an Escherichia coli O157:H7 outbreak associated with undercooked ground beef were characterized by pulsed-field gel electrophoresis and Shiga-like toxin genotype. Pulsed-field gel electrophoresis confirmed the epidemiologically implicated source of the two-state outbreak and differentiated between outbreak and sporadic strains.
摘要
从一起与未煮熟的碎牛肉相关的大肠杆菌O157:H7疫情中分离出的食品和患者菌株，通过脉冲场凝胶电泳和志贺样毒素基因型进行了特征分析。脉冲场凝胶电泳证实了这起涉及两个州的疫情在流行病学上的相关源头，并区分了疫情菌株和散发菌株。
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