• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

脉冲场凝胶电泳技术在食源性大肠杆菌O157:H7暴发流行期间的流行病学研究中的应用

Use of pulsed-field gel electrophoresis for epidemiological study of Escherichia coli O157:H7 during a food-borne outbreak.

作者信息

Johnson J M, Weagant S D, Jinneman K C, Bryant J L

机构信息

Seattle District Laboratory, Food and Drug Administration, Bothell, Washington 98041, USA.

出版信息

Appl Environ Microbiol. 1995 Jul;61(7):2806-8. doi: 10.1128/aem.61.7.2806-2808.1995.

DOI:10.1128/aem.61.7.2806-2808.1995
PMID:7618896
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC167556/
Abstract

Food and patient isolates from an Escherichia coli O157:H7 outbreak associated with undercooked ground beef were characterized by pulsed-field gel electrophoresis and Shiga-like toxin genotype. Pulsed-field gel electrophoresis confirmed the epidemiologically implicated source of the two-state outbreak and differentiated between outbreak and sporadic strains.

摘要

从一起与未煮熟的碎牛肉相关的大肠杆菌O157:H7疫情中分离出的食品和患者菌株，通过脉冲场凝胶电泳和志贺样毒素基因型进行了特征分析。脉冲场凝胶电泳证实了这起涉及两个州的疫情在流行病学上的相关源头，并区分了疫情菌株和散发菌株。

相似文献

1
Use of pulsed-field gel electrophoresis for epidemiological study of Escherichia coli O157:H7 during a food-borne outbreak.脉冲场凝胶电泳技术在食源性大肠杆菌O157:H7暴发流行期间的流行病学研究中的应用
Appl Environ Microbiol. 1995 Jul;61(7):2806-8. doi: 10.1128/aem.61.7.2806-2808.1995.
2
Genetic heterogeneity of Escherichia coli O157:H7 in Scotland and its utility in strain subtyping.苏格兰大肠杆菌O157:H7的遗传异质性及其在菌株分型中的应用。
Eur J Clin Microbiol Infect Dis. 1998 Dec;17(12):844-8. doi: 10.1007/s100960050204.
3
Molecular epidemiology of a fast-food restaurant-associated outbreak of Escherichia coli O157:H7 in Washington State.华盛顿州一家快餐店相关的大肠杆菌O157:H7疫情的分子流行病学研究
J Clin Microbiol. 1995 Aug;33(8):2155-8. doi: 10.1128/jcm.33.8.2155-2158.1995.
4
Molecular epidemiology of Escherichia coli O157:H7 by restriction fragment length polymorphism using Shiga-like toxin genes.利用志贺样毒素基因通过限制性片段长度多态性对大肠杆菌O157:H7进行分子流行病学研究
J Clin Microbiol. 1995 Aug;33(8):2150-4. doi: 10.1128/jcm.33.8.2150-2154.1995.
5
DNA fingerprinting of Escherichia coli O157:H7 strains by pulsed-field gel electrophoresis.通过脉冲场凝胶电泳对大肠杆菌O157:H7菌株进行DNA指纹分析。
J Clin Microbiol. 1992 Aug;30(8):2169-72. doi: 10.1128/jcm.30.8.2169-2172.1992.
6
Laboratory investigation of a multistate food-borne outbreak of Escherichia coli O157:H7 by using pulsed-field gel electrophoresis and phage typing.通过脉冲场凝胶电泳和噬菌体分型对多州食源性大肠杆菌O157:H7疫情进行实验室调查。
J Clin Microbiol. 1994 Dec;32(12):3013-7. doi: 10.1128/jcm.32.12.3013-3017.1994.
7
Detection, isolation, and molecular subtyping of Escherichia coli O157:H7 and Campylobacter jejuni associated with a large waterborne outbreak.与一次大规模水源性暴发相关的大肠杆菌O157:H7和空肠弯曲菌的检测、分离及分子分型
J Clin Microbiol. 2003 Jan;41(1):174-80. doi: 10.1128/JCM.41.1.174-180.2003.
8
High genomic diversity of enterohemorrhagic Escherichia coli isolates in Japan and its applicability for the detection of diffuse outbreak.日本肠出血性大肠杆菌分离株的高基因组多样性及其在检测广泛爆发中的适用性。
Jpn J Infect Dis. 2002 Feb;55(1):19-22.
9
Outbreak of Shiga-toxigenic Escherichia coli O157:H7 infections associated with rodeo attendance, Utah and Idaho, 2009.2009 年，犹他州和爱达荷州因参加牛仔竞技表演而爆发的产志贺毒素大肠杆菌 O157:H7 感染。
Foodborne Pathog Dis. 2011 Oct;8(10):1131-3. doi: 10.1089/fpd.2011.0884. Epub 2011 Jun 9.
10
Multistate Outbreak of Escherichia coli O157:H7 Infections Associated with Consumption of Fresh Spinach: United States, 2006.2006年美国与食用新鲜菠菜相关的大肠杆菌O157:H7感染多州暴发
J Food Prot. 2016 Dec;79(12):2024-2030. doi: 10.4315/0362-028X.JFP-15-556.

引用本文的文献

1
Whole-Genome Sequencing and Bioinformatic Analysis of Environmental, Agricultural, and Human Isolates From East Tennessee.田纳西州东部环境、农业和人类分离株的全基因组测序与生物信息学分析
Front Microbiol. 2020 Nov 5;11:571064. doi: 10.3389/fmicb.2020.571064. eCollection 2020.
2
The EpiQuant Framework for Computing Epidemiological Concordance of Microbial Subtyping Data.用于计算微生物分型数据流行病学一致性的EpiQuant框架。
J Clin Microbiol. 2017 May;55(5):1334-1349. doi: 10.1128/JCM.01945-16. Epub 2017 Feb 15.
3
Polymorphic Amplified Typing Sequences (PATS) Strain Typing System Accurately Discriminates a Set of Temporally and Spatially Disparate Escherichia coli O157 Isolates Associated with Human Infection.多态性扩增分型序列（PATS）菌株分型系统准确区分了一组与人类感染相关的、在时间和空间上分布不同的大肠杆菌O157分离株。
Open Microbiol J. 2013 Oct 31;7:123-9. doi: 10.2174/1874285801307010123. eCollection 2013.
4
Polymorphic Amplified Typing Sequences and Pulsed-Field Gel Electrophoresis Yield Comparable Results in the Strain Typing of a Diverse Set of Bovine Escherichia coli O157:H7 Isolates.多态性扩增分型序列和脉冲场凝胶电泳在多种牛源大肠杆菌O157:H7分离株的菌株分型中产生可比结果。
Int J Microbiol. 2012;2012:140105. doi: 10.1155/2012/140105. Epub 2012 Aug 7.
5
Enteric viruses of humans and animals in aquatic environments: health risks, detection, and potential water quality assessment tools.水生环境中人类和动物的肠道病毒：健康风险、检测及潜在的水质评估工具
Microbiol Mol Biol Rev. 2005 Jun;69(2):357-71. doi: 10.1128/MMBR.69.2.357-371.2005.
6
Escherichia coli O157:H7 outbreak associated with consumption of ground beef, June-July 2002.2002年6月至7月，与食用碎牛肉相关的大肠杆菌O157:H7暴发。
Public Health Rep. 2005 Mar-Apr;120(2):174-8. doi: 10.1177/003335490512000211.
7
Development of a rapid PCR method using the insertion sequence IS1203 for genotyping Shiga toxin-producing Escherichia coli O157.利用插入序列IS1203开发一种用于产志贺毒素大肠杆菌O157基因分型的快速聚合酶链反应方法。
J Clin Microbiol. 2004 Dec;42(12):5462-6. doi: 10.1128/JCM.42.12.5462-5466.2004.
8
Development of a PCR-restriction fragment length polymorphism assay for the epidemiological analysis of Shiga toxin-producing Escherichia coli.用于产志贺毒素大肠杆菌流行病学分析的聚合酶链反应-限制性片段长度多态性检测方法的建立。
J Clin Microbiol. 2004 Nov;42(11):5205-13. doi: 10.1128/JCM.42.11.5205-5213.2004.
9
Insertions, deletions, and single-nucleotide polymorphisms at rare restriction enzyme sites enhance discriminatory power of polymorphic amplified typing sequences, a novel strain typing system for Escherichia coli O157:H7.在稀有限制性酶切位点的插入、缺失和单核苷酸多态性增强了多态性扩增分型序列的鉴别能力，这是一种用于大肠杆菌O157:H7的新型菌株分型系统。
J Clin Microbiol. 2004 Jun;42(6):2388-97. doi: 10.1128/JCM.42.6.2388-2397.2004.
10
Evaluation of pulsed-field gel electrophoresis as a tool for determining the degree of genetic relatedness between strains of Escherichia coli O157:H7.评估脉冲场凝胶电泳作为确定大肠杆菌O157:H7菌株间遗传相关性程度的工具。
J Clin Microbiol. 2003 May;41(5):1843-9. doi: 10.1128/JCM.41.5.1843-1849.2003.

本文引用的文献

1
An outbreak of diarrhea and hemolytic uremic syndrome from Escherichia coli O157:H7 in fresh-pressed apple cider.新鲜压榨苹果汁中大肠杆菌O157:H7引发的腹泻和溶血性尿毒综合征疫情。
JAMA. 1993 May 5;269(17):2217-20.
2
Comparison and genomic sizing of Escherichia coli O157:H7 isolates by pulsed-field gel electrophoresis.通过脉冲场凝胶电泳对大肠杆菌O157:H7分离株进行比较和基因组大小测定。
Appl Environ Microbiol. 1993 Sep;59(9):3141-4. doi: 10.1128/aem.59.9.3141-3144.1993.
3
A swimming-associated outbreak of hemorrhagic colitis caused by Escherichia coli O157:H7 and Shigella sonnei.由大肠杆菌O157:H7和宋内志贺菌引起的与游泳相关的出血性结肠炎暴发。
N Engl J Med. 1994 Sep 1;331(9):579-84. doi: 10.1056/NEJM199409013310904.
4
Comparison of restriction endonuclease analysis, ribotyping, and pulsed-field gel electrophoresis for molecular differentiation of Clostridium difficile strains.用于艰难梭菌菌株分子鉴别分析的限制性内切酶分析、核糖体分型和脉冲场凝胶电泳方法的比较
J Clin Microbiol. 1994 Aug;32(8):1963-9. doi: 10.1128/jcm.32.8.1963-1969.1994.
5
Molecular epidemiology of Escherichia coli O157:H7 strains by bacteriophage lambda restriction fragment length polymorphism analysis: application to a multistate foodborne outbreak and a day-care center cluster.通过噬菌体λ限制性片段长度多态性分析对大肠杆菌O157:H7菌株进行分子流行病学研究：应用于多州食源性疾病暴发和日托中心聚集性病例
J Clin Microbiol. 1993 Dec;31(12):3179-83. doi: 10.1128/jcm.31.12.3179-3183.1993.
6
Laboratory investigation of a multistate food-borne outbreak of Escherichia coli O157:H7 by using pulsed-field gel electrophoresis and phage typing.通过脉冲场凝胶电泳和噬菌体分型对多州食源性大肠杆菌O157:H7疫情进行实验室调查。
J Clin Microbiol. 1994 Dec;32(12):3013-7. doi: 10.1128/jcm.32.12.3013-3017.1994.
7
Phage-typing scheme for Escherichia coli O157:H7.大肠杆菌O157:H7的噬菌体分型方案。
J Infect Dis. 1987 Apr;155(4):806-9. doi: 10.1093/infdis/155.4.806.
8
Genetic evidence of clonal descent of Escherichia coli O157:H7 associated with hemorrhagic colitis and hemolytic uremic syndrome.与出血性结肠炎和溶血尿毒综合征相关的大肠杆菌O157:H7克隆谱系的遗传学证据。
J Infect Dis. 1988 Jun;157(6):1124-33. doi: 10.1093/infdis/157.6.1124.
9
Toxin genotypes and plasmid profiles as determinants of systemic sequelae in Escherichia coli O157:H7 infections.毒素基因型和质粒图谱作为大肠杆菌O157:H7感染全身后遗症的决定因素。
J Infect Dis. 1989 Dec;160(6):994-8. doi: 10.1093/infdis/160.6.994.
10
Infection by verocytotoxin-producing Escherichia coli.产志贺毒素大肠杆菌感染
Clin Microbiol Rev. 1989 Jan;2(1):15-38. doi: 10.1128/CMR.2.1.15.