Influence of type of diet and incubation time on in sacco release of Cu, Fe, Mn and Zn from Italian ryegrass, untreated and ammonia treated wheat straw.

Artificially dried ryegrass, untreated and ammonia-treated wheat straw were ground and incubated in nylon bags in the rumen of three sheep each fed with diets based on roughage or concentrate. Dry matter degradability, the concentration and the release of the trace elements Cu, Fe, Mn and Zn from the incubated feeds were measured after 0 (washing loss), 6, 12, 24, 48 and 72 h rumen incubation time. Dry matter degradability, trace element concentration and their release were significantly influenced by the kind of incubated feeds, incubation time and feeding of sheep. Cu- (1.8-6.9 mg kg-1 DM) and Zn concentrations (36-103 mg kg-1 DM) of straw residues in the bags were much higher than those of original straw (1.2-1.6 and 8.1-9.9 mg kg-1 DM resp.). The inflow of Cu and Zn in the bags containing straw residues was higher than their release. The Cu-, Fe- and Mn-release from ryegrass was similar to the dry matter degradability, but the Zn-release was much lower.