人回肠脂质结合蛋白的克隆与染色体定位

Cloning and chromosomal localization of the human ileal lipid-binding protein.

作者信息

Oelkers P, Dawson P A

机构信息

Department of Internal Medicine-Gastroenterology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, NC 27157, USA.

出版信息

Biochim Biophys Acta. 1995 Jul 13;1257(2):199-202. doi: 10.1016/0005-2760(95)00098-w.

DOI:10.1016/0005-2760(95)00098-w

PMID:7619861

Abstract

A human ileal lipid-binding protein (ILBP3) cDNA was isolated and shown to encode a 128 amino acid protein with homology to the fatty acid-binding protein gene family. The human ILBP amino acid sequence exhibited 78% identity to the rat ILBP sequence. Northern blot analysis revealed a single transcript of approx. 0.6 kb in small intestine, but no hybridization to liver, spleen, thymus, prostate, ovary, caecum, or colon. Southern blot analysis of genomic DNA from a panel of rodent-human somatic cell hybrids revealed that the human ILBP gene resides on chromosome 5 and is not linked to any other known fatty acid-binding protein family member.

摘要

一个人回肠脂质结合蛋白（ILBP3）cDNA被分离出来，显示其编码一个由128个氨基酸组成的蛋白质，与脂肪酸结合蛋白基因家族具有同源性。人ILBP氨基酸序列与大鼠ILBP序列有78%的同一性。Northern印迹分析显示在小肠中有一个约0.6 kb的单一转录本，但在肝脏、脾脏、胸腺、前列腺、卵巢、盲肠或结肠中没有杂交信号。对一组啮齿动物-人类体细胞杂种的基因组DNA进行Southern印迹分析表明，人ILBP基因位于5号染色体上，且不与任何其他已知的脂肪酸结合蛋白家族成员连锁。